| Host: |
Rabbit |
| Applications: |
WB |
| Reactivity: |
Human/Mouse |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: |
Rabbit polyclonal antibody anti-DGKZ (808-1117) is suitable for use in Western Blot research applications. |
| Clonality: |
Polyclonal |
| Conjugation: |
Unconjugated |
| Isotype: |
IgG |
| Formulation: |
PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3. |
| Purification: |
Affinity purification |
| Dilution Range: |
WB 1:500-1:2000 |
| Storage Instruction: |
Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: |
DGKZ |
| Gene ID: |
8525 |
| Uniprot ID: |
DGKZ_HUMAN |
| Immunogen Region: |
808-1117 |
| Immunogen: |
Recombinant fusion protein containing a sequence corresponding to amino acids 808-1117 of human DGKZ (NP_001099010.1). |
| Immunogen Sequence: |
ATMVQKAKRRSAAPLHSDQQ PVPEQLRIQVSRVSMHDYEA LHYDKEQLKEASVPLGTVVV PGDSDLELCRAHIERLQQEP DGAGAKSPTCQKLSPKWCFL DATTASRFYRIDRAQEHLNY VTEIAQDEIYILDPELLGAS ARPDLPTPTSPLPTSPCSPT PRSLQGDAAPPQGEELIEAA KRNDFCKLQELHRAGGDLMH RDEQSRTLLHHAVSTGSKDV VRYLLDHAPPEILDAVEEN |
| Tissue Specificity | Highest levels in brain, and substantial levels in skeletal muscle, heart, and pancreas. Isoform 2: Predominantly expressed in muscle. |
| Post Translational Modifications | Phosphorylation of the MARCKS homology domain by PKC reduces nuclear accumulation of DGK-zeta. |
| Function | Diacylglycerol kinase that converts diacylglycerol/DAG into phosphatidic acid/phosphatidate/PA and regulates the respective levels of these two bioactive lipids. Thereby, acts as a central switch between the signaling pathways activated by these second messengers with different cellular targets and opposite effects in numerous biological processes. Also plays an important role in the biosynthesis of complex lipids (Probable). Does not exhibit an acyl chain-dependent substrate specificity among diacylglycerol species. Can also phosphorylate 1-alkyl-2-acylglycerol in vitro but less efficiently and with a preference for alkylacylglycerols containing an arachidonoyl group. The biological processes it is involved in include T cell activation since it negatively regulates T-cell receptor signaling which is in part mediated by diacylglycerol. By generating phosphatidic acid, stimulates PIP5KIA activity which regulates actin polymerization. Through the same mechanism could also positively regulate insulin-induced translocation of SLC2A4 to the cell membrane. Isoform 1: Regulates RASGRP1 activity. Isoform 2: Does not regulate RASGRP1 activity. |
| Protein Name | Diacylglycerol Kinase ZetaDag Kinase ZetaDiglyceride Kinase ZetaDgk-Zeta |
| Database Links | Reactome: R-HSA-114508 |
| Cellular Localisation | NucleusCytoplasmCytosolCell MembraneCell ProjectionLamellipodium |
| Alternative Antibody Names | Anti-Diacylglycerol Kinase Zeta antibodyAnti-Dag Kinase Zeta antibodyAnti-Diglyceride Kinase Zeta antibodyAnti-Dgk-Zeta antibodyAnti-DGKZ antibodyAnti-DAGK6 antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
