| Host: |
Rabbit |
| Applications: |
WB/IHC/IP |
| Reactivity: |
Human/Mouse/Rat |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: |
Rabbit polyclonal antibody anti-DGCR8 (180-340) is suitable for use in Western Blot, Immunohistochemistry and Immunoprecipitation research applications. |
| Clonality: |
Polyclonal |
| Conjugation: |
Unconjugated |
| Isotype: |
IgG |
| Formulation: |
PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3. |
| Purification: |
Affinity purification |
| Dilution Range: |
WB 1:500-1:2000IHC-P 1:50-1:200IP 1:500-1:1000 |
| Storage Instruction: |
Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: |
DGCR8 |
| Gene ID: |
54487 |
| Uniprot ID: |
DGCR8_HUMAN |
| Immunogen Region: |
180-340 |
| Immunogen: |
Recombinant fusion protein containing a sequence corresponding to amino acids 180-340 of human DGCR8 (NP_073557.3). |
| Immunogen Sequence: |
DKKDEENELDQEKRVEYAVL DELEDFTDNLELDEEGAGGF TAKAIVQRDRVDEEALNFPY EDDFDNDVDALLEEGLCAPK KRRTEEKYGGDSDHPSDGET SVQPMMTKIKTVLKSRGRPP TEPLPDGWIMTFHNSGVPVY LHRESRVVTWSRPYFLGTGS I |
| Tissue Specificity | Ubiquitously expressed. |
| Function | Component of the microprocessor complex that acts as a RNA- and heme-binding protein that is involved in the initial step of microRNA (miRNA) biogenesis. Component of the microprocessor complex that is required to process primary miRNA transcripts (pri-miRNAs) to release precursor miRNA (pre-miRNA) in the nucleus. Within the microprocessor complex, DGCR8 function as a molecular anchor necessary for the recognition of pri-miRNA at dsRNA-ssRNA junction and directs DROSHA to cleave 11 bp away form the junction to release hairpin-shaped pre-miRNAs that are subsequently cut by the cytoplasmic DICER to generate mature miRNAs. The heme-bound DGCR8 dimer binds pri-miRNAs as a cooperative trimer (of dimers) and is active in triggering pri-miRNA cleavage, whereas the heme-free DGCR8 monomer binds pri-miRNAs as a dimer and is much less active. Both double-stranded and single-stranded regions of a pri-miRNA are required for its binding. Specifically recognizes and binds N6-methyladenosine (m6A)-containing pri-miRNAs, a modification required for pri-miRNAs processing. Involved in the silencing of embryonic stem cell self-renewal. |
| Protein Name | Microprocessor Complex Subunit Dgcr8Digeorge Syndrome Critical Region 8 |
| Database Links | Reactome: R-HSA-203927Reactome: R-HSA-8986944 |
| Cellular Localisation | NucleusNucleolusColocalizes With Nucleolin And Drosha In The NucleolusMostly Detected In The Nucleolus As Electron-Dense Granular Patches Around The Fibrillar Center (Fc) And Granular Component (Gc)Also Detected In The Nucleoplasm As Small Foci Adjacent To Splicing Speckles Near The Chromatin StructureLocalized With Drosha In Gw Bodies (Gwbs)Also Known As P-Bodies |
| Alternative Antibody Names | Anti-Microprocessor Complex Subunit Dgcr8 antibodyAnti-Digeorge Syndrome Critical Region 8 antibodyAnti-DGCR8 antibodyAnti-C22orf12 antibodyAnti-DGCRK6 antibodyAnti-LP4941 antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
