| Short Description : | Rabbit polyclonal DFFB (109-338) antibody for use in WB, IHC-P and ELISA in human and mouse samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications: | WB/IHC-P/ELISA |
| Host: | Rabbit |
| Reactivity: | Human/Mouse |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Clonality : | Polyclonal |
| Isotype: | IgG |
| Conjugation: | Unconjugated |
| Concentration: | Lot specific |
| Purification: | Affinity purification |
| Dilution Range: | WB:1:200-1:1000IHC-P:1:50-1:200ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Formulation: | PBS with 0.02% Sodium Azide, 50% Glycerol, pH 7.3. |
| Storage Instruction: | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: | DFFB |
| Gene ID: | 1677 |
| Uniprot ID: | DFFB_HUMAN |
| Immunogen Region: | 109-338 |
| Immunogen Sequence: | DEQAPQRQRLLADLLHNVSQ NIAAETRAEDPPWFEGLESR FQSKSGYLRYSCESRIRSYL REVSSYPSTVGAEAQEEFLR VLGSMCQRLRSMQYNGSYFD RGAKGGSRLCTPEGWFSCQG PFDMDSCLSRHSINPYSNRE SRILFSTWNLDHIIEKKRTI IPTLVEAIKEQDGREVDWEY FYGLLFTSENLKLVHIVCHK KTTHKLNCDPSRIYKPQTRL KRKQPVRKRQ |
| Specificity: | Recombinant fusion protein containing a sequence corresponding to amino acids 109-338 of human DFFB (NP_004393.1). |
| Function | Nuclease that induces DNA fragmentation and chromatin condensation during apoptosis. Degrades naked DNA and induces apoptotic morphology. |
| Protein Name | Dna Fragmentation Factor Subunit BetaCaspase-Activated DeoxyribonucleaseCadCaspase-Activated DnaseCaspase-Activated NucleaseCpanDna Fragmentation Factor 40 Kda SubunitDff-40 |
| Database Links | Reactome: R-HSA-140342 |
| Cellular Localisation | CytoplasmNucleus |
| Alternative Antibody Names | Anti-Dna Fragmentation Factor Subunit Beta antibodyAnti-Caspase-Activated Deoxyribonuclease antibodyAnti-Cad antibodyAnti-Caspase-Activated Dnase antibodyAnti-Caspase-Activated Nuclease antibodyAnti-Cpan antibodyAnti-Dna Fragmentation Factor 40 Kda Subunit antibodyAnti-Dff-40 antibodyAnti-DFFB antibodyAnti-CAD antibodyAnti-DFF2 antibodyAnti-DFF40 antibody |
Information sourced from Uniprot.org
![Immunofluorescence analysis of U-2 OS cells using [KO Validated] TFAP4 Rabbit polyclonal antibody (STJ119138) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/600x533/products/98011/381531/STJ119138_1__83947.1713149676.jpg?c=1 "Immunofluorescence analysis of U-2 OS cells using [KO Validated] TFAP4 Rabbit polyclonal antibody (STJ119138) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.")
