Western blot analysis of extracts of various cell lines, using Destrin Rabbit monoclonal antibody (STJ11103125) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.

Anti-Destrin antibody (1-100) [S5MR] (STJ11103125)

⇓ Datasheet ⇓ SDS
SPECIFICATIONS
ClonalityMonoclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
STJ11103125
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General Information

Short DescriptionRabbit monoclonal Destrin (1-100) antibody for use in WB and ELISA in human, mouse and rat samples. Datasheet included with dilution recommendations, and related reagents.
ApplicationsWB/ELISA
HostRabbit
ReactivityHuman/Mouse/Rat
NoteSTRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.

Product Properties

ClonalityMonoclonal
Clone IDS5MR
IsotypeIgG
ConjugationUnconjugated
ConcentrationLot specific
PurificationAffinity purification
Dilution RangeWB:1:500-1:1000
ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements.
FormulationPBS with 0.02% Sodium Azide, 0.05% BSA, 50% Glycerol, pH 7.3.
Storage InstructionStore at-20ยฐC for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.

Target Information

Gene SymbolDSTN
Gene ID11034
Uniprot IDDEST_HUMAN
Immunogen Region1-100
Immunogen SequenceMASGVQVADEVCRIFYDMKV RKCSTPEEIKKRKKAVIFCL SADKKCIIVEEGKEILVGDV GVTITDPFKHFVGMLPEKDC RYALYDASFETKESRKEELM
SpecificityA synthetic peptide corresponding to a sequence within amino acids 1-100 of human Destrin (P60981).

Additional Info

Tissue Specificity Widely distributed in various tissues.
Post Translational Modifications ISGylated.
Function Actin-depolymerizing protein. Severs actin filaments (F-actin) and binds to actin monomers (G-actin). Acts in a pH-independent manner.
Protein Name Destrin
Actin-Depolymerizing Factor
Adf
Database Links
Cellular Localisation
Alternative Antibody Names Anti-Destrin antibody
Anti-Actin-Depolymerizing Factor antibody
Anti-Adf antibody
Anti-DSTN antibody
Anti-ACTDP antibody
Anti-DSN antibody

Information sourced from Uniprot.org

Citations

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