| Host: |
Rabbit |
| Applications: |
WB |
| Reactivity: |
Human/Mouse/Rat |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: |
Rabbit polyclonal antibody anti-DAP3-binding cell death enhancer 1 is suitable for use in Western Blot research applications. |
| Clonality: |
Polyclonal |
| Conjugation: |
Unconjugated |
| Isotype: |
IgG |
| Formulation: |
Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide. |
| Purification: |
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Concentration: |
1 mg/mL |
| Dilution Range: |
WB 1:500-2000 |
| Storage Instruction: |
Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: |
DELE1 |
| Gene ID: |
9812 |
| Uniprot ID: |
DELE1_HUMAN |
| Specificity: |
This antibody detects endogenous levels of DELE1 at Human, Mouse, Rat |
| Immunogen: |
Synthesized peptide derived from human DELE1 |
| Post Translational Modifications | DAP3-binding cell death enhancer 1: Unstable protein in absence of stress: imported in the mitochondrial matrix following processing by the mitochondrial-processing peptidase (MPP), where it is degraded by LONP1. Stabilized in response to iron deficiency: iron deficiency impairs mitochondrial import, promoting localization at the mitochondrial surface and stabilization. Cleaved by OMA1 in response to mitochondrial stress, generating the DAP3-binding cell death enhancer 1 short form (DELE1(S) or S-DELE1) that accumulates in the cytosol and activates the protein kinase activity of EIF2AK1/HRI. Protein cleavage by OMA1 can take place at different positions, and apparently does not require a specific sequence motif. DAP3-binding cell death enhancer 1 short form: Ubiquitinated and degraded by the SIFI complex once the mitochondrial stress has been resolved, thereby providing stress response silencing. Within the SIFI complex, UBR4 initiates ubiquitin chain that are further elongated or branched by KCMF1. |
| Function | Protein kinase activator that acts as a key activator of the integrated stress response (ISR) following various stresses, such as iron deficiency, mitochondrial stress or mitochondrial DNA breaks. Detects impaired protein import and processing in mitochondria, activating the ISR. May also required for the induction of death receptor-mediated apoptosis through the regulation of caspase activation. DAP3-binding cell death enhancer 1: Protein kinase activator that activates the ISR in response to iron deficiency: iron deficiency impairs mitochondrial import, promoting DELE1 localization at the mitochondrial surface, where it binds and activates EIF2AK1/HRI to trigger the ISR. DAP3-binding cell death enhancer 1 short form: Protein kinase activator generated by protein cleavage in response to mitochondrial stress, which accumulates in the cytosol and specifically binds to and activates the protein kinase activity of EIF2AK1/HRI. It thereby activates the integrated stress response (ISR): EIF2AK1/HRI activation promotes eIF-2-alpha (EIF2S1) phosphorylation, leading to a decrease in global protein synthesis and the induction of selected genes, including the transcription factor ATF4, the master transcriptional regulator of the ISR. Also acts as an activator of PRKN-independent mitophagy: activates the protein kinase activity of EIF2AK1/HRI in response to mitochondrial damage, promoting eIF-2-alpha (EIF2S1) phosphorylation, leading to mitochondrial localization of EIF2S1 followed by induction of mitophagy. |
| Protein Name | Dap3-Binding Cell Death Enhancer 1Dap3-Binding Cell Death Enhancer 1 - Long FormDele1(LDeath Ligand Signal Enhancer Cleaved Into - Dap3-Binding Cell Death Enhancer 1 Short FormDele1(SS-Dele1Cdele1 |
| Database Links | Reactome: R-HSA-9840373 |
| Cellular Localisation | Dap3-Binding Cell Death Enhancer 1: MitochondrionMitochondrion Outer MembraneMitochondrion Inner MembraneImported In The Mitochondrial Matrix In Absence Of StressLeading To Its Degradation By Lonp1Localizes At The Mitochondrial Surface In Response To Iron Deficiency: Iron Deficiency Impairs Mitochondrial ImportPromoting Localization At The Mitochondrial Surface And StabilizationAssociates With The Mitochondrion Inner Membrane In Response To Mitochondrial StressLeading To Its Proteolytic Processing By Oma1And Generation Of The Ap3-Binding Cell Death Enhancer 1 Short Form (Dele1(S) Or S-Dele1)Dap3-Binding Cell Death Enhancer 1 Short Form: CytoplasmCytosolThis Short Form Is Generated By Proteolytic Processing By Oma1 In Response To Mitochondrial StressLeading To Translocation To The Cytosol |
| Alternative Antibody Names | Anti-Dap3-Binding Cell Death Enhancer 1 antibodyAnti-Dap3-Binding Cell Death Enhancer 1 - Long Form antibodyAnti-Dele1(L antibodyAnti-Death Ligand Signal Enhancer Cleaved Into - Dap3-Binding Cell Death Enhancer 1 Short Form antibodyAnti-Dele1(S antibodyAnti-S-Dele1 antibodyAnti-Cdele1 antibodyAnti-DELE1 antibodyAnti-DELE antibodyAnti-KIAA0141 antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
