• Western blot analysis of extracts of various cell lines, using DDX39A antibody (STJ110264) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.
  • Immunohistochemistry analysis of paraffin-embedded mouse kidney using DDX39A antibody (STJ110264) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of paraffin-embedded rat brain using DDX39A antibody (STJ110264) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of paraffin-embedded human stomach using DDX39A antibody (STJ110264) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunofluorescence analysis of U2OS cells using DDX39A antibody (STJ110264) at dilution of 1:50. Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of NIH/3T3 cells using DDX39A antibody (STJ110264) at dilution of 1:50. Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of PC-12 cells using DDX39A antibody (STJ110264) at dilution of 1:50. Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of NIH/3T3 cells using DDX39A rabbit polyclonal antibody (STJ110264) at dilution of 1:50 (40x lens). Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of PC-12 cells using DDX39A rabbit polyclonal antibody (STJ110264) at dilution of 1:50 (40x lens). Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of U2OS cells using DDX39A rabbit polyclonal antibody (STJ110264) at dilution of 1:50 (40x lens). Blue: DAPI for nuclear staining.

Anti-DDX39A antibody (1-100) (STJ110264)

SKU:
STJ110264

£169.50 - £449.50
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Host: Rabbit
Applications: WB/IHC/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-DDX39A (1-100) is suitable for use in Western Blot, Immunohistochemistry and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.05% Proclin300, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:1000
IHC-P 1:50-1:200
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: DDX39A
Gene ID: 10212
Uniprot ID: DX39A_HUMAN
Immunogen Region: 1-100
Immunogen: A synthetic peptide corresponding to a sequence within amino acids 1-100 of human DDX39A (NP_005795.2).
Immunogen Sequence: MAEQDVENDLLDYDEEEEPQ APQESTPAPPKKDIKGSYVS IHSSGFRDFLLKPELLRAIV DCGFEHPSEVQHECIPQAIL GMDVLCQAKSGMGKTAVFVL
Tissue Specificity Detected in testis, and at lower levels in brain, kidney, lung, thymus, spleen and salivary gland.
Function Isoform 1: Involved in pre-mRNA splicing. Required for the export of mRNA out of the nucleus.
Protein Name Atp-Dependent Rna Helicase Ddx39a
Dead Box Protein 39
Nuclear Rna Helicase Urh49
Database Links Reactome: R-HSA-159236
Reactome: R-HSA-72187
Reactome: R-HSA-73856
Cellular Localisation Nucleus
Cytoplasm
Can Translocate To The Cytoplasm In The Presence Of Mx1
Alternative Antibody Names Anti-Atp-Dependent Rna Helicase Ddx39a antibody
Anti-Dead Box Protein 39 antibody
Anti-Nuclear Rna Helicase Urh49 antibody
Anti-DDX39A antibody
Anti-DDX39 antibody

Information sourced from Uniprot.org

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