• Immunofluorescence analysis of NIH-3T3 cells using DDAH1 Rabbit polyclonal antibody (STJ112333) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
  • Western blot analysis of various lysates using DDAH1 Rabbit polyclonal antibody (STJ112333) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.

Anti-DDAH1 antibody (1-285) (STJ112333)

SKU:
STJ112333

£112.50 - £522.50
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Host: Rabbit
Applications: WB/IF/IP
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-DDAH1 (1-285) is suitable for use in Western Blot, Immunofluorescence and Immunoprecipitation research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IF/ICC 1:50-1:200
IP 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: DDAH1
Gene ID: 23576
Uniprot ID: DDAH1_HUMAN
Immunogen Region: 1-285
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-285 of human DDAH1 (NP_036269.1).
Immunogen Sequence: MAGLGHPAAFGRATHAVVRA LPESLGQHALRSAKGEEVDV ARAERQHQLYVGVLGSKLGL QVVELPADESLPDCVFVEDV AVVCEETALITRPGAPSRRK EVDMMKEALEKLQLNIVEMK DENATLDGGDVLFTGREFFV GLSKRTNQRGAEILADTFKD YAVSTVPVADGLHLKSFCSM AGPNLIAIGSSESAQKALKI MQQMSDHRYDKLTVPDDIAA NCIYLNIPNKGHVLLHRTP
Tissue Specificity Detected in brain, liver, kidney and pancreas, and at low levels in skeletal muscle.
Function Hydrolyzes N(G),N(G)-dimethyl-L-arginine (ADMA) and N(G)-monomethyl-L-arginine (MMA) which act as inhibitors of NOS. Has therefore a role in the regulation of nitric oxide generation.
Protein Name N(G -N(G-Dimethylarginine Dimethylaminohydrolase 1
Ddah-1
Dimethylarginine Dimethylaminohydrolase 1
Ddahi
Dimethylargininase-1
Database Links Reactome: R-HSA-203615
Alternative Antibody Names Anti-N(G -N(G-Dimethylarginine Dimethylaminohydrolase 1 antibody
Anti-Ddah-1 antibody
Anti-Dimethylarginine Dimethylaminohydrolase 1 antibody
Anti-Ddahi antibody
Anti-Dimethylargininase-1 antibody
Anti-DDAH1 antibody
Anti-DDAH antibody

Information sourced from Uniprot.org

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