• Western blot analysis of extracts of various cell lines, using DAZL antibody (STJ115905) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.
  • Immunohistochemistry analysis of paraffin-embedded rat testis using DAZL rabbit polyclonal antibody (STJ115905) at dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of paraffin-embedded mouse testis using DAZL rabbit polyclonal antibody (STJ115905) at dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Immunofluorescence analysis of rat testis using DAZL rabbit polyclonal antibody (STJ115905) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of mouse testis using DAZL rabbit polyclonal antibody (STJ115905) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Anti-DAZL antibody (200-295) (STJ115905)

SKU:
STJ115905

£169.50 - £449.50
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Host: Rabbit
Applications: WB/IHC/IF
Reactivity: Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-DAZL (200-295) is suitable for use in Western Blot, Immunohistochemistry and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:1000
IHC-P 1:50-1:200
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: DAZL
Gene ID: 1618
Uniprot ID: DAZL_HUMAN
Immunogen Region: 200-295
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 200-295 of human DAZL (NP_001342.2).
Immunogen Sequence: QRSYVVPPAYSAVNYHCNEV DPGAEVVPNECSVHEATPPS GNGPQKKSVDR
Tissue Specificity Testis specific.
Function RNA-binding protein, which is essential for gametogenesis in both males and females. Plays a central role during spermatogenesis. Acts by binding to the 3'-UTR of mRNA, specifically recognizing GUU triplets, and thereby regulating the translation of key transcripts.
Protein Name Deleted In Azoospermia-Like
Daz Homolog
Daz-Like Autosomal
Deleted In Azoospermia-Like 1
Spgy-Like-Autosomal
Cellular Localisation Cytoplasm
Nucleus
Predominantly Cytoplasmic
Nuclear In Spermatogonia Until Near The End Of The Meiotic Prophase And Cytoplasmic Localization From Then Onward
Alternative Antibody Names Anti-Deleted In Azoospermia-Like antibody
Anti-Daz Homolog antibody
Anti-Daz-Like Autosomal antibody
Anti-Deleted In Azoospermia-Like 1 antibody
Anti-Spgy-Like-Autosomal antibody
Anti-DAZL antibody
Anti-DAZH antibody
Anti-DAZL1 antibody
Anti-DAZLA antibody
Anti-SPGYLA antibody

Information sourced from Uniprot.org

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