• Western blot analysis of extracts of various cell lines, using DARPP32 Rabbit monoclonal antibody (STJ11103546) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.

Anti-DARPP32 antibody (1-100) [S6MR] (STJ11103546)

SKU:
STJ11103546

Shipping:
Free Shipping
Current Stock:
Host: Rabbit
Applications: WB
Reactivity: Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit monoclonal antibody anti-DARPP32 (1-100) is suitable for use in Western Blot research applications.
Clonality: Monoclonal
Clone ID: S6MR
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 0.05% BSA, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: PPP1R1B
Gene ID: 84152
Uniprot ID: PPR1B_HUMAN
Immunogen Region: 1-100
Immunogen: A synthetic peptide corresponding to a sequence within amino acids 1-100 of human DARPP32 (Q9UD71).
Immunogen Sequence: MDPKDRKKIQFSVPAPPSQL DPRQVEMIRRRRPTPAMLFR LSEHSSPEEEASPHQRASGE GHHLKSKRPNPCAYTPPSLK AVQRIAESHLQSISNLNENQ
Post Translational Modifications Dopamine- and cyclic AMP-regulated neuronal phosphoprotein. Phosphorylation of Thr-34 is required for activity.
Function Inhibitor of protein-phosphatase 1.
Protein Name Protein Phosphatase 1 Regulatory Subunit 1b
Darpp-32
Dopamine- And Camp-Regulated Neuronal Phosphoprotein
Database Links Reactome: R-HSA-180024
Cellular Localisation Cytoplasm
Alternative Antibody Names Anti-Protein Phosphatase 1 Regulatory Subunit 1b antibody
Anti-Darpp-32 antibody
Anti-Dopamine- And Camp-Regulated Neuronal Phosphoprotein antibody
Anti-PPP1R1B antibody
Anti-DARPP32 antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance