| Host: | Rabbit |
| Applications: | WB/ICC/IF/IP |
| Reactivity: | Human/Mouse/Rat |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description : | Rabbit monoclonal anti-Cytochrome C for use in WB, ICC, IF and IP in Human, Mouse and Rat samples. Datasheet included with dilution recommendations, and related reagents. |
| Clonality : | Monoclonal |
| Clone ID : | R02-9A7 |
| Conjugation: | Unconjugated |
| Isotype: | IgG |
| Formulation: | 50mM Tris-Glycine (pH 7.4) , 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA |
| Purification: | Affinity Purified |
| Concentration: | 0.3 mg/mL |
| Dilution Range: | WB 1:500-1:1000IF 1:50-1:200IP 1:20 |
| Storage Instruction: | Store at 4°C short term. Aliquot and store at-20°C long term. Avoid freeze/thaw cycles. |
| Gene Symbol: | CYCS |
| Gene ID: | 54205 |
| Uniprot ID: | CYC_HUMAN |
| Immunogen: | A synthetic peptide of human Cytochrome C |
| Post Translational Modifications | Binds 1 heme c group covalently per subunit. Phosphorylation at Tyr-49 and Tyr-98 both reduce by half the turnover in the reaction with cytochrome c oxidase, down-regulating mitochondrial respiration. |
| Function | Electron carrier protein. The oxidized form of the cytochrome c heme group can accept an electron from the heme group of the cytochrome c1 subunit of cytochrome reductase. Cytochrome c then transfers this electron to the cytochrome oxidase complex, the final protein carrier in the mitochondrial electron-transport chain. Plays a role in apoptosis. Suppression of the anti-apoptotic members or activation of the pro-apoptotic members of the Bcl-2 family leads to altered mitochondrial membrane permeability resulting in release of cytochrome c into the cytosol. Binding of cytochrome c to Apaf-1 triggers the activation of caspase-9, which then accelerates apoptosis by activating other caspases. |
| Protein Name | Cytochrome C |
| Database Links | Reactome: R-HSA-111457Reactome: R-HSA-111458Reactome: R-HSA-111459Reactome: R-HSA-111463Reactome: R-HSA-111464Reactome: R-HSA-2151201Reactome: R-HSA-3299685Reactome: R-HSA-5620971Reactome: R-HSA-5628897Reactome: R-HSA-611105Reactome: R-HSA-9627069Reactome: R-HSA-9707564 |
| Cellular Localisation | Mitochondrion Intermembrane SpaceLoosely Associated With The Inner Membrane |
| Alternative Antibody Names | Anti-Cytochrome C antibodyAnti-CYCS antibodyAnti-CYC antibody |
Information sourced from Uniprot.org