| Host: |
Rabbit |
| Applications: |
WB/IHC/IF/ELISA |
| Reactivity: |
Human/Mouse |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: |
Rabbit polyclonal antibody anti-7-alpha-hydroxycholest-4-en-3-one 12-alpha-hydroxylase (371-420 aa) is suitable for use in Western Blot, Immunohistochemistry, Immunofluorescence and ELISA research applications. |
| Clonality: |
Polyclonal |
| Conjugation: |
Unconjugated |
| Isotype: |
IgG |
| Formulation: |
Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide. |
| Purification: |
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Concentration: |
1 mg/mL |
| Dilution Range: |
WB 1:500-1:2000IHC 1:100-1:300ELISA 1:40000IF 1:50-200 |
| Storage Instruction: |
Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: |
CYP8B1 |
| Gene ID: |
1582 |
| Uniprot ID: |
CP8B1_HUMAN |
| Immunogen Region: |
371-420 aa |
| Specificity: |
CYP8B1 Polyclonal Antibody detects endogenous levels of CYP8B1 protein. |
| Immunogen: |
The antiserum was produced against synthesized peptide derived from the human Cytochrome P450 8B1 at the amino acid range 371-420 |
| Function | A cytochrome P450 monooxygenase involved in primary bile acid biosynthesis. Catalyzes the 12alpha-hydroxylation of 7alpha-hydroxy-4-cholesten-3-one, an intermediate metabolite in cholic acid biosynthesis. Controls biliary balance of cholic acid and chenodeoxycholic acid, ultimately regulating the intestinal absorption of dietary lipids. Mechanistically, uses molecular oxygen inserting one oxygen atom into a substrate, and reducing the second into a water molecule, with two electrons provided by NADPH via cytochrome P450 reductase (CPR.NADPH--hemoprotein reductase). |
| Protein Name | 7-Alpha-Hydroxycholest-4-En-3-One 12-Alpha-Hydroxylase7-Alpha-Hydroxy-4-Cholesten-3-One 12-Alpha-HydroxylaseCypviiib1Cytochrome P450 8b1Sterol 12-Alpha-Hydroxylase |
| Database Links | Reactome: R-HSA-193368Reactome: R-HSA-193775Reactome: R-HSA-193807Reactome: R-HSA-197264Reactome: R-HSA-211979Reactome: R-HSA-211994Reactome: R-HSA-2162123 |
| Cellular Localisation | Endoplasmic Reticulum MembraneSingle-Pass Membrane ProteinMicrosome Membrane |
| Alternative Antibody Names | Anti-7-Alpha-Hydroxycholest-4-En-3-One 12-Alpha-Hydroxylase antibodyAnti-7-Alpha-Hydroxy-4-Cholesten-3-One 12-Alpha-Hydroxylase antibodyAnti-Cypviiib1 antibodyAnti-Cytochrome P450 8b1 antibodyAnti-Sterol 12-Alpha-Hydroxylase antibodyAnti-CYP8B1 antibodyAnti-CYP12 antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
