Western blot analysis of various lysates, using CYP4V2 antibody (STJ28656) at 1:900 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.

Anti-CYP4V2 antibody (206-525) (STJ28656)

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STJ28656
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Short Description :Rabbit polyclonal CYP4V2 (206-525) antibody for use in WB, IF, ICC and ELISA in human, mouse and rat samples. Datasheet included with dilution recommendations, and related reagents.
Applications:WB/IF/ICC/ELISA
Host:Rabbit
Reactivity:Human/Mouse/Rat
Note:STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Clonality :Polyclonal
Isotype:IgG
Conjugation:Unconjugated
Concentration:Lot specific
Purification:Affinity purification
Dilution Range:WB:1:500-1:1000
IF/ICC:1:50-1:200
ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements.
Formulation:PBS with 0.05% Proclin300, 50% Glycerol, pH7.3.
Storage Instruction:Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol:CYP4V2
Gene ID:285440
Uniprot ID:CP4V2_HUMAN
Immunogen Region:206-525
Immunogen Sequence:ICETAMGKNIGAQSNDDSEY VRAVYRMSEMIFRRIKMPWL WLDLWYLMFKEGWEHKKSLQ ILHTFTNSVIAERANEMNAN EDCRGDGRGSAPSKNKRRAF LDLLLSVTDDEGNRLSHEDI REEVDTFMFEGHDTTAAAIN WSLYLLGSNPEVQKKVDHEL DDVFGKSDRPATVEDLKKLR YLECVIKETLRLFPSVPLFA RSVSEDCEVAGYRVLKGTEA VIIPYALHRDPRYFPNPEE
Specificity:A synthetic peptide corresponding to a sequence within amino acids 200-300 of human CYP4V2 (NP_997235.3).
Tissue Specificity Broadly expressed. Detected in heart, brain, placenta, lung, liver, skeletal muscle, kidney, pancreas, retina, retinal pigment epithelium (RPE) and lymphocytes.
Function A cytochrome P450 monooxygenase involved in fatty acid metabolism in the eye. Catalyzes the omega-hydroxylation of polyunsaturated fatty acids (PUFAs) docosahexaenoate (DHA) and its precursor eicosapentaenoate (EPA), and may contribute to the homeostasis of these retinal PUFAs. Omega hydroxylates saturated fatty acids such as laurate, myristate and palmitate, the catalytic efficiency decreasing in the following order: myristate > laurate > palmitate (C14>C12>C16). Mechanistically, uses molecular oxygen inserting one oxygen atom into a substrate, and reducing the second into a water molecule, with two electrons provided by NADPH via cytochrome P450 reductase (CPR.NADPH-ferrihemoprotein reductase).
Protein Name Cytochrome P450 4v2
Docosahexaenoic Acid Omega-Hydroxylase Cyp4v2
Long-Chain Fatty Acid Omega-Monooxygenase
Database Links Reactome: R-HSA-211976
Reactome: R-HSA-2453902
Cellular Localisation Endoplasmic Reticulum Membrane
Single-Pass Membrane Protein
Alternative Antibody Names Anti-Cytochrome P450 4v2 antibody
Anti-Docosahexaenoic Acid Omega-Hydroxylase Cyp4v2 antibody
Anti-Long-Chain Fatty Acid Omega-Monooxygenase antibody
Anti-CYP4V2 antibody

Information sourced from Uniprot.org

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