| Short Description : | Rabbit polyclonal CYP4F12 (265-524) antibody for use in WB and ELISA in human, mouse and rat samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications: | WB/ELISA |
| Host: | Rabbit |
| Reactivity: | Human/Mouse/Rat |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Clonality : | Polyclonal |
| Isotype: | IgG |
| Conjugation: | Unconjugated |
| Concentration: | Lot specific |
| Purification: | Affinity purification |
| Dilution Range: | WB:1:500-1:2000ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Formulation: | PBS with 0.02% Sodium Azide, 50% Glycerol, pH 7.3. |
| Storage Instruction: | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: | CYP4F12 |
| Gene ID: | 66002 |
| Uniprot ID: | CP4FC_HUMAN |
| Immunogen Region: | 265-524 |
| Immunogen Sequence: | DFTDAVIRERRRTLPTQGID DFFKDKAKSKTLDFIDVLLL SKDEDGKALSDEDIRAEADT FMFGGHDTTASGLSWVLYNL ARHPEYQERCRQEVQELLKD RDPKEIEWDDLAQLPFLTMC VKESLRLHPPAPFISRCCTQ DIVLPDGRVIPKGITCLIDI IGVHHNPTVWPDPEVYDPFR FDPENSKGRSPLAFIPFSAG PRNCIGQAFAMAEMKVVLAL MLLHFRFLPDHTEPRRKLE |
| Specificity: | Recombinant fusion protein containing a sequence corresponding to amino acids 265-524 of human CYP4F12 (NP_076433.3). |
| Tissue Specificity | Expressed in small intestine, liver, colon and heart. |
| Function | A cytochrome P450 monooxygenase involved in the metabolism of endogenous polyunsaturated fatty acids (PUFAs). Mechanistically, uses molecular oxygen inserting one oxygen atom into a substrate, and reducing the second into a water molecule, with two electrons provided by NADPH via cytochrome P450 reductase (CPR.NADPH-ferrihemoprotein reductase). Catalyzes the hydroxylation of carbon hydrogen bonds, with preference for omega-2 position. Metabolizes (5Z,8Z,11Z,14Z)-eicosatetraenoic acid (arachidonate) toward 18-hydroxy arachidonate. Catalyzes the epoxidation of double bonds of PUFAs such as docosapentaenoic and docosahexaenoic acids. Has low omega-hydroxylase activity toward leukotriene B4 and arachidonate. Involved in the metabolism of xenobiotics. Catalyzes the hydroxylation of the antihistamine drug ebastine. |
| Protein Name | Cytochrome P450 4f12Cypivf12 |
| Database Links | Reactome: R-HSA-211935Reactome: R-HSA-211979 |
| Cellular Localisation | Endoplasmic Reticulum MembraneMicrosome Membrane |
| Alternative Antibody Names | Anti-Cytochrome P450 4f12 antibodyAnti-Cypivf12 antibodyAnti-CYP4F12 antibodyAnti-UNQ568 antibodyAnti-PRO1129 antibody |
Information sourced from Uniprot.org
