Anti-CYP46A1 antibody (400-500) (STJ11105570)
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
General Information
| Short Description | Rabbit polyclonal Cholesterol 24-Hydroxylase (400-500) antibody for use in WB, IF, ICC and ELISA in mouse and rat samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB/IF/ICC/ELISA |
| Host | Rabbit |
| Reactivity | Mouse/Rat |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | Lot specific |
| Purification | Affinity purification |
| Dilution Range | WB:1:500-1:1000IF/ICC:1:50-1:200ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Formulation | PBS with 0.05% Proclin300, 50% Glycerol, pH 7.3. |
| Storage Instruction | Store at-20ยฐC for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | CYP46A1 |
| Gene ID | 10858 |
| Uniprot ID | CP46A_HUMAN |
| Immunogen Region | 400-500 |
| Immunogen Sequence | RMDTYFEDPLTFNPDRFGPG APKPRFTYFPFSLGHRSCIG QQFAQMEVKVVMAKLLQRLE FRLVPGQRFGLQEQATLKPL DPVLCTLRPRGWQPAPPPPP C |
| Specificity | A synthetic peptide corresponding to a sequence within amino acids 400-500 of human CYP46A1 (NP_006659.1). |
Additional Info
| Tissue Specificity | Expressed in brain. The mRNA was broadly distributed with higher levels in gray matter zones and lower levels in regions rich in white matter. Not detected in fetal sample but its expression increases linearly with age. |
| Function | P450 monooxygenase that plays a major role in cholesterol homeostasis in the brain. Primarily catalyzes the hydroxylation (with S stereochemistry) at C-24 of cholesterol side chain, triggering cholesterol diffusion out of neurons and its further degradation. By promoting constant cholesterol elimination in neurons, may activate the mevalonate pathway and coordinate the synthesis of new cholesterol and nonsterol isoprenoids involved in synaptic activity and learning. Further hydroxylates cholesterol derivatives and hormone steroids on both the ring and side chain of these molecules, converting them into active oxysterols involved in lipid signaling and biosynthesis. Acts as an epoxidase converting cholesta-5,24-dien-3beta-ol/desmosterol into (24S),25-epoxycholesterol, an abundant lipid ligand of nuclear NR1H2 and NR1H3 receptors shown to promote neurogenesis in developing brain. May also catalyze the oxidative metabolism of xenobiotics, such as clotrimazole. |
| Protein Name | Cholesterol 24-HydroxylaseCh24hCholesterol 24-MonooxygenaseCholesterol 24s-HydroxylaseCytochrome P450 46a1 |
| Database Links | Reactome: R-HSA-193775Reactome: R-HSA-211976 |
| Cellular Localisation | Endoplasmic Reticulum MembraneSingle-Pass Membrane ProteinMicrosome MembranePostsynapsePresynapseCell ProjectionDendrite |
| Alternative Antibody Names | Anti-Cholesterol 24-Hydroxylase antibodyAnti-Ch24h antibodyAnti-Cholesterol 24-Monooxygenase antibodyAnti-Cholesterol 24s-Hydroxylase antibodyAnti-Cytochrome P450 46a1 antibodyAnti-CYP46A1 antibodyAnti-CYP46 antibody |
Information sourced from Uniprot.org