• Western blot analysis of lysates from COS7 cells, treated with Forskolin 40nM 30', using Cyclin D3 Antibody. The lane on the right is blocked with the synthesized peptide.
  • Immunohistochemical analysis of paraffin-embedded Human tonsil. Antibody was diluted at 1:100 (4°C overnight). High-pressure and temperature Tris-EDTA, pH8.0 was used for antigen retrieval. Negetive contrl (right) obtaned from antibody was pre-absorbed by immunogen peptide.
  • Immunohistochemistry analysis of paraffin-embedded human lung carcinoma tissue, using Cyclin D3 Antibody. The picture on the right is blocked with the synthesized peptide.
  • Western blot analysis of COS7 cells using Cyclin D3 Polyclonal Antibody

Anti-CCND3 antibody (243-292 aa) (STJ92540)

SKU:
STJ92540

£46.00 - £226.00
Current Stock:
Host: Rabbit
Applications: WB/IHC/IF/ELISA
Reactivity: Human/Mouse/Rat/Monkey
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-G1/S-specific cyclin-D3 (243-292 aa) is suitable for use in Western Blot, Immunohistochemistry, Immunofluorescence and ELISA research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide.
Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Concentration: 1 mg/mL
Dilution Range: WB 1:500-1:2000
IHC 1:100-1:300
ELISA 1:5000
IF 1:50-200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: CCND3
Gene ID: 896
Uniprot ID: CCND3_HUMAN
Immunogen Region: 243-292 aa
Specificity: Cyclin D3 Polyclonal Antibody detects endogenous levels of Cyclin D3 protein.
Immunogen: The antiserum was produced against synthesized peptide derived from the human Cyclin D3 at the amino acid range 243-292
Function Regulatory component of the cyclin D3-CDK4 (DC) complex that phosphorylates and inhibits members of the retinoblastoma (RB) protein family including RB1 and regulates the cell-cycle during G(1)/S transition. Phosphorylation of RB1 allows dissociation of the transcription factor E2F from the RB/E2F complex and the subsequent transcription of E2F target genes which are responsible for the progression through the G(1) phase. Hypophosphorylates RB1 in early G(1) phase. Cyclin D-CDK4 complexes are major integrators of various mitogenenic and antimitogenic signals. Component of the ternary complex, cyclin D3/CDK4/CDKN1B, required for nuclear translocation and activity of the cyclin D-CDK4 complex. Shows transcriptional coactivator activity with ATF5 independently of CDK4.
Protein Name G1/S-Specific Cyclin-D3
Database Links Reactome: R-HSA-381340
Reactome: R-HSA-5687128
Reactome: R-HSA-69231
Reactome: R-HSA-8934593
Reactome: R-HSA-9661069
Reactome: R-HSA-9754119
Cellular Localisation Nucleus
Cytoplasm
Alternative Antibody Names Anti-G1/S-Specific Cyclin-D3 antibody
Anti-CCND3 antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance