• Western blot analysis of RAW264. 7, using CYBA antibody (STJ116414) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.
  • Western blot analysis of Rat lung, using CYBA antibody (STJ116414) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.
  • Immunofluorescence analysis of MCF7 cells using CYBA rabbit polyclonal antibody (STJ116414) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of NIH/3T3 cells using CYBA rabbit polyclonal antibody (STJ116414) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of PC-12 cells using CYBA rabbit polyclonal antibody (STJ116414) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Anti-CYBA antibody (126-195) (STJ116414)

SKU:
STJ116414

£112.50 - £522.50
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Host: Rabbit
Applications: WB/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-CYBA (126-195) is suitable for use in Western Blot and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:1000
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: CYBA
Gene ID: 1535
Uniprot ID: CY24A_HUMAN
Immunogen Region: 126-195
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 126-195 of human CYBA (NP_000092.2).
Immunogen Sequence: VRGEQWTPIEPKPRERPQIG GTIKQPPSNPPPRPPAEARK KPSEEEAAVAAGGPPGGPQV NPIPVTDEVV
Post Translational Modifications The heme prosthetic group could be coordinated with residues of the light chain, the heavy chain, or both, and it is possible that more than one heme is present per cytochrome b-245. Phosphorylation at Thr-147 enhances NADPH oxidase activity by promoting p47phox binding. Ubiquitinated at Lys-149 likely by RNF145.
Function Critical component of the membrane-bound oxidase of phagocytes that generates superoxide. Associates with NOX3 to form a functional NADPH oxidase constitutively generating superoxide.
Protein Name Cytochrome B-245 Light Chain
Cytochrome B(558 Alpha Chain
Cytochrome B558 Subunit Alpha
Neutrophil Cytochrome B 22 Kda Polypeptide
Superoxide-Generating Nadph Oxidase Light Chain Subunit
P22 Phagocyte B-Cytochrome
P22-Phox
P22phox
Database Links Reactome: R-HSA-1222556
Reactome: R-HSA-1236973
Reactome: R-HSA-3299685
Reactome: R-HSA-4420097
Reactome: R-HSA-5668599
Reactome: R-HSA-6798695
Reactome: R-HSA-9013149
Reactome: R-HSA-9013404
Reactome: R-HSA-9013423
Reactome: R-HSA-9673324
Cellular Localisation Cell Membrane
As Unassembled Monomer May Localize To The Endoplasmic Reticulum
Alternative Antibody Names Anti-Cytochrome B-245 Light Chain antibody
Anti-Cytochrome B(558 Alpha Chain antibody
Anti-Cytochrome B558 Subunit Alpha antibody
Anti-Neutrophil Cytochrome B 22 Kda Polypeptide antibody
Anti-Superoxide-Generating Nadph Oxidase Light Chain Subunit antibody
Anti-P22 Phagocyte B-Cytochrome antibody
Anti-P22-Phox antibody
Anti-P22phox antibody
Anti-CYBA antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance