• Immunofluorescence analysis of U-2 OS cells using PGRMC1 Rabbit polyclonal antibody (STJ27586) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of L929 cells using PGRMC1 Rabbit polyclonal antibody (STJ27586) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of C6 cells using PGRMC1 Rabbit polyclonal antibody (STJ27586) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
  • Immunohistochemistry analysis of PGRMC1 in paraffin-embedded mouse brain using PGRMC1 Rabbit polyclonal antibody (STJ27586) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of PGRMC1 in paraffin-embedded human vermiform appendix using PGRMC1 Rabbit polyclonal antibody (STJ27586) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of PGRMC1 in paraffin-embedded human tonsil using PGRMC1 Rabbit polyclonal antibody (STJ27586) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of PGRMC1 in paraffin-embedded rat kidney using PGRMC1 Rabbit polyclonal antibody (STJ27586) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Western blot analysis of various lysates using PGRMC1 Rabbit polyclonal antibody (STJ27586) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 90s.

Anti-PGRMC1 antibody (44-195) (STJ27586)

SKU:
STJ27586

£169.50 - £449.50
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Host: Rabbit
Applications: WB/IHC/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-PGRMC1 (44-195) is suitable for use in Western Blot, Immunohistochemistry and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IHC-P 1:50-1:100
IF/ICC 1:50-1:100
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: PGRMC1
Gene ID: 10857
Uniprot ID: PGRC1_HUMAN
Immunogen Region: 44-195
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 44-195 of human PGRMC1 (NP_006658.1).
Immunogen Sequence: KIVRGDQPAASGDSDDDEPP PLPRLKRRDFTPAELRRFDG VQDPRILMAINGKVFDVTKG RKFYGPEGPYGVFAGRDASR GLATFCLDKEALKDEYDDLS DLTAAQQETLSDWESQFTFK YHHVGKLLKEGEEPTVYSDE EEPKDESARKND
Tissue Specificity Detected in urine (at protein level). Expressed by endometrial glands and stroma (at protein level). Widely expressed, with highest expression in liver and kidney.
Post Translational Modifications O-glycosylated.contains chondroitin sulfate attached to Ser-54. Ser-54 is in the cytoplasmic domain but the glycosylated form was detected in urine, suggesting that the membrane-bound form is cleaved, allowing for production of a secreted form which is glycosylated.
Function Component of a progesterone-binding protein complex. Binds progesterone. Has many reported cellular functions (heme homeostasis, interaction with CYPs). Required for the maintenance of uterine histoarchitecture and normal female reproductive lifespan. Intracellular heme chaperone. Regulates heme synthesis via interactions with FECH and acts as a heme donor for at least some hemoproteins.
Protein Name Membrane-Associated Progesterone Receptor Component 1
Mpr
Dap1
Iza
Database Links Reactome: R-HSA-6798695
Cellular Localisation Microsome Membrane
Single-Pass Membrane Protein
Smooth Endoplasmic Reticulum Membrane
Mitochondrion Outer Membrane
Extracellular Side
Secreted
Alternative Antibody Names Anti-Membrane-Associated Progesterone Receptor Component 1 antibody
Anti-Mpr antibody
Anti-Dap1 antibody
Anti-Iza antibody
Anti-PGRMC1 antibody
Anti-HPR6.6 antibody
Anti-PGRMC antibody

Information sourced from Uniprot.org

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