Anti-CYBA antibody (126-195) (STJ116414)
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
General Information
| Short Description | Rabbit polyclonal CYBA (126-195) antibody for use in WB, IF, ICC and ELISA in human, mouse and rat samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB/IF/ICC/ELISA |
| Host | Rabbit |
| Reactivity | Human/Mouse/Rat |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | Lot specific |
| Purification | Affinity purification |
| Dilution Range | WB:1:500-1:1000IF/ICC:1:50-1:200ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Formulation | PBS with 0.02% Sodium Azide, 50% Glycerol, pH 7.3. |
| Storage Instruction | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | CYBA |
| Gene ID | 1535 |
| Uniprot ID | CY24A_HUMAN |
| Immunogen Region | 126-195 |
| Immunogen Sequence | VRGEQWTPIEPKPRERPQIG GTIKQPPSNPPPRPPAEARK KPSEEEAAVAAGGPPGGPQV NPIPVTDEVV |
| Specificity | Recombinant fusion protein containing a sequence corresponding to amino acids 126-195 of human CYBA (NP_000092.2). |
Additional Info
| Post Translational Modifications | Phosphorylation at Thr-147 enhances NADPH oxidase activity by promoting NCF1/p47-phox binding. Ubiquitinated at Lys-149 likely by RNF145. |
| Function | Subunit of NADPH oxidase complexes that is required for the NADPH oxidase activity that generates, in various cell types, superoxide from molecular oxygen utilizing NADPH as an electron donor. Subunit of the phagocyte NADPH oxidase complex that mediates the transfer of electrons from cytosolic NADPH to O2 to produce the superoxide anion (O2(-)). In the activated complex, electrons are first transferred from NADPH to flavin adenine dinucleotide (FAD) and subsequently transferred via two heme molecules to molecular oxygen, producing superoxide through an outer-sphere reaction. Activation of the NADPH oxidase complex is initiated by the assembly of cytosolic subunits of the NADPH oxidase complex with the core NADPH oxidase complex to form a complex at the plasma membrane or phagosomal membrane. This activation process is initiated by phosphorylation dependent binding of the cytosolic NCF1/p47-phox subunit to the C-terminus of CYBA/p22-phox. Aassociates with NOX3 to form a functional NADPH oxidase constitutively generating superoxide. |
| Protein Name | Cytochrome B-245 Light ChainCytochrome B(558 Alpha ChainCytochrome B558 Subunit AlphaNeutrophil Cytochrome B 22 Kda PolypeptideSuperoxide-Generating Nadph Oxidase Light Chain SubunitP22 Phagocyte B-CytochromeP22-PhoxP22phox |
| Database Links | Reactome: R-HSA-1222556Reactome: R-HSA-1236973Reactome: R-HSA-3299685Reactome: R-HSA-4420097Reactome: R-HSA-5668599Reactome: R-HSA-6798695Reactome: R-HSA-9013149Reactome: R-HSA-9013404Reactome: R-HSA-9013423Reactome: R-HSA-9673324 |
| Cellular Localisation | Cell MembraneMulti-Pass Membrane Protein |
| Alternative Antibody Names | Anti-Cytochrome B-245 Light Chain antibodyAnti-Cytochrome B(558 Alpha Chain antibodyAnti-Cytochrome B558 Subunit Alpha antibodyAnti-Neutrophil Cytochrome B 22 Kda Polypeptide antibodyAnti-Superoxide-Generating Nadph Oxidase Light Chain Subunit antibodyAnti-P22 Phagocyte B-Cytochrome antibodyAnti-P22-Phox antibodyAnti-P22phox antibodyAnti-CYBA antibody |
Information sourced from Uniprot.org