| Host: |
Rabbit |
| Applications: |
WB/IHC/IF |
| Reactivity: |
Human/Mouse/Rat |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: |
Rabbit polyclonal antibody anti-CUL4B (1-200) is suitable for use in Western Blot, Immunohistochemistry and Immunofluorescence research applications. |
| Clonality: |
Polyclonal |
| Conjugation: |
Unconjugated |
| Isotype: |
IgG |
| Formulation: |
PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3. |
| Purification: |
Affinity purification |
| Dilution Range: |
WB 1:500-1:2000IHC-P 1:50-1:200IF/ICC 1:50-1:200 |
| Storage Instruction: |
Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: |
CUL4B |
| Gene ID: |
8450 |
| Uniprot ID: |
CUL4B_HUMAN |
| Immunogen Region: |
1-200 |
| Immunogen: |
Recombinant fusion protein containing a sequence corresponding to amino acids 1-200 of human CUL4B (NP_003579.3). |
| Immunogen Sequence: |
MMSQSSGSGDGNDDEATTSK DGGFSSPSPSAAAAAQEVRS ATDGNTSTTPPTSAKKRKLN SSSSSSSNSSNEREDFDSTS SSSSTPPLQPRDSASPSTSS FCLGVSVAASSHVPIQKKLR FEDTLEFVGFDAKMAEESSS SSSSSSPTAATSQQQQLKNK SILISSVASVHHANGLAKSS TTVSSFANSKPGSAKKLVIK |
| Post Translational Modifications | Neddylated. Deneddylated via its interaction with the COP9 signalosome (CSN) complex. |
| Function | Core component of multiple cullin-RING-based E3 ubiquitin-protein ligase complexes which mediate the ubiquitination and subsequent proteasomal degradation of target proteins. The functional specificity of the E3 ubiquitin-protein ligase complex depends on the variable substrate recognition subunit. CUL4B may act within the complex as a scaffold protein, contributing to catalysis through positioning of the substrate and the ubiquitin-conjugating enzyme. Plays a role as part of the E3 ubiquitin-protein ligase complex in polyubiquitination of CDT1, histone H2A, histone H3 and histone H4 in response to radiation-induced DNA damage. Targeted to UV damaged chromatin by DDB2 and may be important for DNA repair and DNA replication. A number of DCX complexes (containing either TRPC4AP or DCAF12 as substrate-recognition component) are part of the DesCEND (destruction via C-end degrons) pathway, which recognizes a C-degron located at the extreme C terminus of target proteins, leading to their ubiquitination and degradation. The DCX(AMBRA1) complex is a master regulator of the transition from G1 to S cell phase by mediating ubiquitination of phosphorylated cyclin-D (CCND1, CCND2 and CCND3). The DCX(AMBRA1) complex also acts as a regulator of Cul5-RING (CRL5) E3 ubiquitin-protein ligase complexes by mediating ubiquitination and degradation of Elongin-C (ELOC) component of CRL5 complexes. Required for ubiquitination of cyclin E (CCNE1 or CCNE2), and consequently, normal G1 cell cycle progression. Regulates the mammalian target-of-rapamycin (mTOR) pathway involved in control of cell growth, size and metabolism. Specific CUL4B regulation of the mTORC1-mediated pathway is dependent upon 26S proteasome function and requires interaction between CUL4B and MLST8. With CUL4A, contributes to ribosome biogenesis. |
| Protein Name | Cullin-4bCul-4b |
| Database Links | Reactome: R-HSA-110314Reactome: R-HSA-5696394Reactome: R-HSA-5696395Reactome: R-HSA-5696400Reactome: R-HSA-6781823Reactome: R-HSA-6781827Reactome: R-HSA-6782135Reactome: R-HSA-6782210Reactome: R-HSA-8951664 |
| Cellular Localisation | CytoplasmNucleusMore Concentrated In Nuclei Than In Cytoplasm In Germinal Vesicle (Gv) Stage OocytesZygotes And The 2-Cell StageBut Distributed In The Cytoplasm At The Mii-Stage Oocytes |
| Alternative Antibody Names | Anti-Cullin-4b antibodyAnti-Cul-4b antibodyAnti-CUL4B antibodyAnti-KIAA0695 antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
