• Immunofluorescence analysis of U2OS cells using CUL4B antibody (STJ27353).
  • Immunofluorescence analysis of U2OS cells using CUL4B antibody (STJ27353). Blue: DAPI for nuclear staining. DNA damage by a UV-A laser.
  • Immunohistochemistry analysis of paraffin-embedded human liver cancer using CUL4B antibody (STJ27353) at dilution of 1:200 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of paraffin-embedded mouse pancreas using CUL4B antibody (STJ27353) at dilution of 1:200 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of paraffin-embedded rat liver using CUL4B antibody (STJ27353) at dilution of 1:200 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Western blot analysis of extracts of various cell lines, using CUL4B antibody (STJ27353) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.

Anti-CUL4B antibody (1-200) (STJ27353)

SKU:
STJ27353

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Host: Rabbit
Applications: WB/IHC/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-CUL4B (1-200) is suitable for use in Western Blot, Immunohistochemistry and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IHC-P 1:50-1:200
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: CUL4B
Gene ID: 8450
Uniprot ID: CUL4B_HUMAN
Immunogen Region: 1-200
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-200 of human CUL4B (NP_003579.3).
Immunogen Sequence: MMSQSSGSGDGNDDEATTSK DGGFSSPSPSAAAAAQEVRS ATDGNTSTTPPTSAKKRKLN SSSSSSSNSSNEREDFDSTS SSSSTPPLQPRDSASPSTSS FCLGVSVAASSHVPIQKKLR FEDTLEFVGFDAKMAEESSS SSSSSSPTAATSQQQQLKNK SILISSVASVHHANGLAKSS TTVSSFANSKPGSAKKLVIK
Post Translational Modifications Neddylated. Deneddylated via its interaction with the COP9 signalosome (CSN) complex.
Function Core component of multiple cullin-RING-based E3 ubiquitin-protein ligase complexes which mediate the ubiquitination and subsequent proteasomal degradation of target proteins. The functional specificity of the E3 ubiquitin-protein ligase complex depends on the variable substrate recognition subunit. CUL4B may act within the complex as a scaffold protein, contributing to catalysis through positioning of the substrate and the ubiquitin-conjugating enzyme. Plays a role as part of the E3 ubiquitin-protein ligase complex in polyubiquitination of CDT1, histone H2A, histone H3 and histone H4 in response to radiation-induced DNA damage. Targeted to UV damaged chromatin by DDB2 and may be important for DNA repair and DNA replication. A number of DCX complexes (containing either TRPC4AP or DCAF12 as substrate-recognition component) are part of the DesCEND (destruction via C-end degrons) pathway, which recognizes a C-degron located at the extreme C terminus of target proteins, leading to their ubiquitination and degradation. The DCX(AMBRA1) complex is a master regulator of the transition from G1 to S cell phase by mediating ubiquitination of phosphorylated cyclin-D (CCND1, CCND2 and CCND3). The DCX(AMBRA1) complex also acts as a regulator of Cul5-RING (CRL5) E3 ubiquitin-protein ligase complexes by mediating ubiquitination and degradation of Elongin-C (ELOC) component of CRL5 complexes. Required for ubiquitination of cyclin E (CCNE1 or CCNE2), and consequently, normal G1 cell cycle progression. Regulates the mammalian target-of-rapamycin (mTOR) pathway involved in control of cell growth, size and metabolism. Specific CUL4B regulation of the mTORC1-mediated pathway is dependent upon 26S proteasome function and requires interaction between CUL4B and MLST8. With CUL4A, contributes to ribosome biogenesis.
Protein Name Cullin-4b
Cul-4b
Database Links Reactome: R-HSA-110314
Reactome: R-HSA-5696394
Reactome: R-HSA-5696395
Reactome: R-HSA-5696400
Reactome: R-HSA-6781823
Reactome: R-HSA-6781827
Reactome: R-HSA-6782135
Reactome: R-HSA-6782210
Reactome: R-HSA-8951664
Cellular Localisation Cytoplasm
Nucleus
More Concentrated In Nuclei Than In Cytoplasm In Germinal Vesicle (Gv) Stage Oocytes
Zygotes And The 2-Cell Stage
But Distributed In The Cytoplasm At The Mii-Stage Oocytes
Alternative Antibody Names Anti-Cullin-4b antibody
Anti-Cul-4b antibody
Anti-CUL4B antibody
Anti-KIAA0695 antibody

Information sourced from Uniprot.org

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