| Host: |
Mouse |
| Applications: |
WB/IHC/IF/ELISA |
| Reactivity: |
Human/Mouse |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: |
Mouse monoclonal antibody anti-Src substrate cortactin is suitable for use in Western Blot, Immunohistochemistry, Immunofluorescence and ELISA research applications. |
| Clonality: |
Monoclonal |
| Clone ID: |
4C6 |
| Conjugation: |
Unconjugated |
| Isotype: |
IgG1 |
| Formulation: |
Liquid in PBS containing 0.03% Sodium Azide, 0.5% BSA, 50% Glycerol. |
| Purification: |
Affinity purification |
| Dilution Range: |
WB 1:500-2000IHC-P 1:100-500IF ICC 1:100-500ELISA 1:5000-20000 |
| Storage Instruction: |
Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: |
CTTN |
| Gene ID: |
2017 |
| Uniprot ID: |
SRC8_HUMAN |
| Specificity: |
Cortactin Monoclonal Antibody detects endogenous levels of Cortactin protein. |
| Immunogen: |
Purified recombinant fragment of human Cortactin expressed in E. Coli. |
| Function | Contributes to the organization of the actin cytoskeleton and cell shape. Plays a role in the formation of lamellipodia and in cell migration. Plays a role in the regulation of neuron morphology, axon growth and formation of neuronal growth cones. Through its interaction with CTTNBP2, involved in the regulation of neuronal spine density. Plays a role in focal adhesion assembly and turnover. In complex with ABL1 and MYLK regulates cortical actin-based cytoskeletal rearrangement critical to sphingosine 1-phosphate (S1P)-mediated endothelial cell (EC) barrier enhancement. Plays a role in intracellular protein transport and endocytosis, and in modulating the levels of potassium channels present at the cell membrane. Plays a role in receptor-mediated endocytosis via clathrin-coated pits. Required for stabilization of KCNH1 channels at the cell membrane. Plays a role in the invasiveness of cancer cells, and the formation of metastases. |
| Protein Name | Src Substrate CortactinAmplaxinOncogene Ems1 |
| Database Links | Reactome: R-HSA-5627123Reactome: R-HSA-8856828 |
| Cellular Localisation | CytoplasmCytoskeletonCell ProjectionLamellipodiumRuffleDendriteCell MembranePeripheral Membrane ProteinCytoplasmic SidePodosomeCell JunctionFocal AdhesionMembraneClathrin-Coated PitDendritic SpineCell CortexEndoplasmic ReticulumColocalizes Transiently With Ptk2/Fak1 At Focal AdhesionsAssociated With Membrane Ruffles And LamellipodiaIn The Presence Of Cttnbp2nlColocalizes With Stress FibersIn The Presence Of Cttnbp2Localizes At The Cell CortexIn Response To Neuronal Activation By GlutamateRedistributes From Dendritic Spines To The Dendritic ShaftColocalizes With Dnm2 At The Basis Of Filopodia In Hippocampus Neuron Growth Zones |
| Alternative Antibody Names | Anti-Src Substrate Cortactin antibodyAnti-Amplaxin antibodyAnti-Oncogene Ems1 antibodyAnti-CTTN antibodyAnti-EMS1 antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
![Western blot analysis of lysates from wild type (WT) and Cortactin knockout (KO) HeLa cells, using [KO Validated] Cortactin Rabbit polyclonal antibody (STJ11100011) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 5s.](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/300x300/products/89084/357544/STJ11100011_1__81515.1713121662.jpg?c=1 "Western blot analysis of lysates from wild type (WT) and Cortactin knockout (KO) HeLa cells, using [KO Validated] Cortactin Rabbit polyclonal antibody (STJ11100011) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 5s.")