• Western blot analysis of Rat brain, using CTNNA3 rabbit monoclonal antibody (STJ11103013) at 1 : 1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3s.
  • Western blot analysis of various lysates, using CTNNA3 rabbit monoclonal antibody (STJ11103013) at 1 : 1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.
  • Immunohistochemistry analysis of paraffin-embedded rat stomach using CTNNA3 rabbit monoclonal antibody (STJ11103013) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9. 0 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of paraffin-embedded human breast cancer using CTNNA3 rabbit monoclonal antibody (STJ11103013) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9. 0 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of paraffin-embedded mouse pancreas using CTNNA3 rabbit monoclonal antibody (STJ11103013) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9. 0 before commencing with immunohistochemistry staining protocol.

Anti-CTNNA3 antibody (700-895) [S3MR] (STJ11103013)

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STJ11103013

£212.50 - £501.50
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Host: Rabbit
Applications: WB/IHC
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit monoclonal antibody anti-CTNNA3 (700-895) is suitable for use in Western Blot and Immunohistochemistry research applications.
Clonality: Monoclonal
Clone ID: S3MR
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 0.05% BSA, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:1000
IHC-P 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: CTNNA3
Gene ID: 29119
Uniprot ID: CTNA3_HUMAN
Immunogen Region: 700-895
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 700-895 of human CTNNA3 (Q9UI47).
Immunogen Sequence: DIIVLAKNMCMIMMEMTDFT RGKGPLKHTTDVIYAAKMIS ESGSRMDVLARQIANQCPDP SCKQDLLAYLEQIKFYSHQL KICSQVKAEIQNLGGELIMS ALDSVTSLIQAAKNLMNAVV QTVKMSYIASTKIIRIQSPA GPRHPVVMWRMKAPAKKPLI KREKPEETCAAVRRGSAKKK IHPLQVMSEFRGRQIY
Tissue Specificity Predominantly expressed in heart and testis. Expressed at lower levels in brain, kidney, liver and skeletal muscle.
Function May be involved in formation of stretch-resistant cell-cell adhesion complexes.
Protein Name Catenin Alpha-3
Alpha T-Catenin
Cadherin-Associated Protein
Cellular Localisation Cytoplasm
Cytoskeleton
Cell Junction
Desmosome
Localizes To Intercalated Disks Of Cardiomyocytes And In Peritubular Myoid Cells Of Testis
And Colocalizes With Ctnna1 And Ctnna2
Colocalizes With Pkp2 At Intercalated Disks In The Heart
Alternative Antibody Names Anti-Catenin Alpha-3 antibody
Anti-Alpha T-Catenin antibody
Anti-Cadherin-Associated Protein antibody
Anti-CTNNA3 antibody

Information sourced from Uniprot.org

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