• Immunofluorescence analysis of Jurkat cells using CTIP2/BCL11B Rabbit polyclonal antibody (STJ11103335) at dilution of 1:50 (40x lens). Blue: DAPI for nuclear staining.
  • Immunohistochemistry analysis of paraffin-embedded rat spleen using CTIP2/BCL11B Rabbit polyclonal antibody (STJ11103335) at dilution of 1:50 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of paraffin-embedded rat brain using CTIP2/BCL11B Rabbit polyclonal antibody (STJ11103335) at dilution of 1:50 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Western blot analysis of extracts of various cell lines, using CTIP2/BCL11B antibody (STJ11103335) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.

Anti-CTIP2/BCL11B antibody (190-310) (STJ11103335)

SKU:
STJ11103335

£169.50 - £449.50
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Host: Rabbit
Applications: WB/IHC/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-CTIP2/BCL11B (190-310) is suitable for use in Western Blot, Immunohistochemistry and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.05% Proclin300, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:1000
IHC-P 1:50-1:200
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: BCL11B
Gene ID: 64919
Uniprot ID: BC11B_HUMAN
Immunogen Region: 190-310
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 190-310 of human CTIP2/BCL11B (NP_612808.1).
Immunogen Sequence: PVSGDGTQGEGQTEAPFGCQ CQLSGKDEPSSYICTTCKQP FNSAWFLLQHAQNTHGFRIY LEPGPASSSLTPRLTIPPPL GPEAVAQSPLMNFLGDSNPF NLLRMTGPILRDHPGFGEGR L
Tissue Specificity Highly expressed in brain and in malignant T-cell lines derived from patients with adult T-cell leukemia/lymphoma.
Post Translational Modifications Sumoylated with SUMO1.
Function Key regulator of both differentiation and survival of T-lymphocytes during thymocyte development in mammals. Essential in controlling the responsiveness of hematopoietic stem cells to chemotactic signals by modulating the expression of the receptors CCR7 and CCR9, which direct the movement of progenitor cells from the bone marrow to the thymus. Is a regulator of IL2 promoter and enhances IL2 expression in activated CD4(+) T-lymphocytes. Tumor-suppressor that represses transcription through direct, TFCOUP2-independent binding to a GC-rich response element. May also function in the P53-signaling pathway.
Protein Name B-Cell Lymphoma/Leukemia 11b
Bcl-11b
B-Cell Cll/Lymphoma 11b
Coup-Tf-Interacting Protein 2
Radiation-Induced Tumor Suppressor Gene 1 Protein
Hrit1
Cellular Localisation Nucleus
Alternative Antibody Names Anti-B-Cell Lymphoma/Leukemia 11b antibody
Anti-Bcl-11b antibody
Anti-B-Cell Cll/Lymphoma 11b antibody
Anti-Coup-Tf-Interacting Protein 2 antibody
Anti-Radiation-Induced Tumor Suppressor Gene 1 Protein antibody
Anti-Hrit1 antibody
Anti-BCL11B antibody
Anti-CTIP2 antibody
Anti-RIT1 antibody

Information sourced from Uniprot.org

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