| Host: | Goat |
| Applications: | Pep-ELISA/WB/IHC |
| Reactivity: | Human/Mouse/Rat/Cow/Dog |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description : | Goat polyclonal antibody anti-CSX1/NKX2-5 (Internal) is suitable for use in ELISA, Western Blot and Immunohistochemistry research applications. |
| Clonality : | Polyclonal |
| Conjugation: | Unconjugated |
| Isotype: | IgG |
| Formulation: | 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. NA |
| Purification: | Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
| Concentration: | 0.5 mg/mL |
| Dilution Range: | Peptide ELISA: antibody detection limit dilution 1:32000.WB: Approx. 38kDa band observed in Mouse Heart lysates (calculated MW of 34.2kDa according to NP_032726.1). Recommended concentration: 1-3µg/ml. Primary incubation 1 hour at room temperatu |
| Storage Instruction: | Store at-20°C on receipt and minimise freeze-thaw cycles. |
| Gene Symbol: | NKX2-5 |
| Gene ID: | 1482 |
| Uniprot ID: | NKX25_HUMAN |
| Immunogen Region: | Internal |
| Accession Number: | NP_004378.1; NP_001159647.1; NP_001159648.1 |
| Specificity: | This antibody is expected to recognize all three reported isoforms (NP_004378.1; NP_001159647.1; NP_001159648.1). |
| Immunogen Sequence: | PRAYSDPDPAKDPR |
| Function | Transcription factor required for the development of the heart and the spleen. During heart development, acts as a transcriptional activator of NPPA/ANF in cooperation with GATA4. May cooperate with TBX2 to negatively modulate expression of NPPA/ANF in the atrioventricular canal. Binds to the core DNA motif of NPPA promoter. Together with PBX1, required for spleen development through a mechanism that involves CDKN2B repression. Positively regulates transcription of genes such as COL3A1 and MMP2, resulting in increased pulmonary endothelial fibrosis in response to hypoxia. |
| Protein Name | Homeobox Protein Nkx-2.5Cardiac-Specific HomeoboxHomeobox Protein CsxHomeobox Protein Nk-2 Homolog E |
| Database Links | Reactome: R-HSA-2032785Reactome: R-HSA-5578768Reactome: R-HSA-9733709 |
| Cellular Localisation | Nucleus |
| Alternative Antibody Names | Anti-Homeobox Protein Nkx-2.5 antibodyAnti-Cardiac-Specific Homeobox antibodyAnti-Homeobox Protein Csx antibodyAnti-Homeobox Protein Nk-2 Homolog E antibodyAnti-NKX2-5 antibodyAnti-CSX antibodyAnti-NKX2.5 antibodyAnti-NKX2E antibody |
Information sourced from Uniprot.org
