• STJ71934 (0. 3µg/ml) staining of Mouse Heart lysate (35µg protein in RIPA buffer). Detected by chemiluminescence.
  • STJ71934 Immunofluorescence analysis of paraformaldehyde fixed U2OS cells, permeabilized with 0. 15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml) , showing nuclear and cytoplasmic staining. The nuclear stain is DAPI (blue). NA NA NA Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).
  • STJ71934 (4µg/ml) staining of paraffin embedded Human Heart. Heat induced antigen retrieval with citrate buffer pH 6, HRP-staining.
  • STJ71934 Negative Control showing staining of paraffin embedded Human Heart, with no primary antibody.

Anti-CSX1/NKX2-5 antibody (Internal) (STJ71934)

SKU:
STJ71934-100

£303.50
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Host: Goat
Applications: Pep-ELISA/WB/IHC
Reactivity: Human/Mouse/Rat/Cow/Dog
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Goat polyclonal antibody anti-CSX1/NKX2-5 (Internal) is suitable for use in ELISA, Western Blot and Immunohistochemistry research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. NA
Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Concentration: 0.5 mg/mL
Dilution Range: IHC-4µg/ml
IF-Strong expression of the protein seen in the nucleus and cytoplasm of U2OS cells. 10µg/ml
ELISA-antibody detection limit dilution 1:32000.
Storage Instruction: Store at-20°C on receipt and minimise freeze-thaw cycles.
Gene Symbol: NKX2-5
Gene ID: 1482
Uniprot ID: NKX25_HUMAN
Immunogen Region: Internal
Accession Number: NP_004378.1; NP_001159647.1; NP_001159648.1
Specificity: This antibody is expected to recognize all three reported isoforms (NP_004378.1; NP_001159647.1; NP_001159648.1).
Immunogen Sequence: PRAYSDPDPAKDPR
Function Transcription factor required for the development of the heart and the spleen. During heart development, acts as a transcriptional activator of NPPA/ANF in cooperation with GATA4. May cooperate with TBX2 to negatively modulate expression of NPPA/ANF in the atrioventricular canal. Binds to the core DNA motif of NPPA promoter. Together with PBX1, required for spleen development through a mechanism that involves CDKN2B repression. Positively regulates transcription of genes such as COL3A1 and MMP2, resulting in increased pulmonary endothelial fibrosis in response to hypoxia.
Protein Name Homeobox Protein Nkx-2.5
Cardiac-Specific Homeobox
Homeobox Protein Csx
Homeobox Protein Nk-2 Homolog E
Database Links Reactome: R-HSA-2032785
Reactome: R-HSA-5578768
Reactome: R-HSA-9733709
Cellular Localisation Nucleus
Alternative Antibody Names Anti-Homeobox Protein Nkx-2.5 antibody
Anti-Cardiac-Specific Homeobox antibody
Anti-Homeobox Protein Csx antibody
Anti-Homeobox Protein Nk-2 Homolog E antibody
Anti-NKX2-5 antibody
Anti-CSX antibody
Anti-NKX2.5 antibody
Anti-NKX2E antibody

Information sourced from Uniprot.org

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