| Host: | Rabbit |
| Applications: | WB/IF/ICC/ELISA |
| Reactivity: | Human/Mouse/Rat |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Clonality : | Polyclonal |
| Conjugation: | Unconjugated |
| Isotype: | IgG |
| Formulation: | PBS with 0.01% Thimerosal, 50% Glycerol, pH 7.3. |
| Purification: | Affinity purification |
| Concentration: | Lot specific |
| Dilution Range: | WB:1:500-1:2000IF/ICC:1:50-1:200ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Storage Instruction: | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: | CRYZ |
| Gene ID: | 1429 |
| Uniprot ID: | QOR_HUMAN |
| Immunogen Region: | 1-329 |
| Specificity: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-329 of human CRYZ (NP_001123514.1). |
| Immunogen Sequence: | MATGQKLMRAVRVFEFGGPE VLKLRSDIAVPIPKDHQVLI KVHACGVNPVETYIRSGTYS RKPLLPYTPGSDVAGVIEAV GDNASAFKKGDRVFTSSTIS GGYAEYALAADHTVYKLPEK LDFKQGAAIGIPYFTAYRAL IHSACVKAGESVLVHGASGG VGLAACQIARAYGLKILGTA GTEEGQKIVLQNGAHEVFNH REVNYIDKIKKYVGEKGIDI IIEMLANVNLSKDLSLLSH |
| Tissue Specificity | Only very low amounts in the lens. |
| Function | Does not have alcohol dehydrogenase activity. Binds NADP and acts through a one-electron transfer process. Orthoquinones, such as 1,2-naphthoquinone or 9,10-phenanthrenequinone, are the best substrates (in vitro). May act in the detoxification of xenobiotics. Interacts with (AU)-rich elements (ARE) in the 3'-UTR of target mRNA species. Enhances the stability of mRNA coding for BCL2. NADPH binding interferes with mRNA binding. |
| Protein Name | Quinone OxidoreductaseNadph -Quinone ReductaseZeta-Crystallin |
| Cellular Localisation | Cytoplasm |
| Alternative Antibody Names | Anti-Quinone Oxidoreductase antibodyAnti-Nadph -Quinone Reductase antibodyAnti-Zeta-Crystallin antibodyAnti-CRYZ antibody |
Information sourced from Uniprot.org
![Western blot analysis of lysates from wild type (WT) and CRYZ knockout (KO) HeLa cells, using [KO Validated] CRYZ Rabbit polyclonal antibody (STJ11100873) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 5s.](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/600x533/products/89807/358274/STJ11100873_1__03594.1713122620.jpg?c=1 "Western blot analysis of lysates from wild type (WT) and CRYZ knockout (KO) HeLa cells, using [KO Validated] CRYZ Rabbit polyclonal antibody (STJ11100873) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 5s.")
