| Host: |
Rabbit |
| Applications: |
WB/IF |
| Reactivity: |
Human/Mouse/Rat |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: |
Rabbit polyclonal antibody anti-CRYZ (1-329) is suitable for use in Western Blot and Immunofluorescence research applications. |
| Clonality: |
Polyclonal |
| Conjugation: |
Unconjugated |
| Isotype: |
IgG |
| Formulation: |
PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3. |
| Purification: |
Affinity purification |
| Dilution Range: |
WB 1:500-1:2000IF/ICC 1:50-1:200 |
| Storage Instruction: |
Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: |
CRYZ |
| Gene ID: |
1429 |
| Uniprot ID: |
QOR_HUMAN |
| Immunogen Region: |
1-329 |
| Immunogen: |
Recombinant fusion protein containing a sequence corresponding to amino acids 1-329 of human CRYZ (NP_001123514.1). |
| Immunogen Sequence: |
MATGQKLMRAVRVFEFGGPE VLKLRSDIAVPIPKDHQVLI KVHACGVNPVETYIRSGTYS RKPLLPYTPGSDVAGVIEAV GDNASAFKKGDRVFTSSTIS GGYAEYALAADHTVYKLPEK LDFKQGAAIGIPYFTAYRAL IHSACVKAGESVLVHGASGG VGLAACQIARAYGLKILGTA GTEEGQKIVLQNGAHEVFNH REVNYIDKIKKYVGEKGIDI IIEMLANVNLSKDLSLLSH |
| Tissue Specificity | Only very low amounts in the lens. |
| Function | Does not have alcohol dehydrogenase activity. Binds NADP and acts through a one-electron transfer process. Orthoquinones, such as 1,2-naphthoquinone or 9,10-phenanthrenequinone, are the best substrates (in vitro). May act in the detoxification of xenobiotics. Interacts with (AU)-rich elements (ARE) in the 3'-UTR of target mRNA species. Enhances the stability of mRNA coding for BCL2. NADPH binding interferes with mRNA binding. |
| Protein Name | Quinone OxidoreductaseNadph -Quinone ReductaseZeta-Crystallin |
| Cellular Localisation | Cytoplasm |
| Alternative Antibody Names | Anti-Quinone Oxidoreductase antibodyAnti-Nadph -Quinone Reductase antibodyAnti-Zeta-Crystallin antibodyAnti-CRYZ antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
![Western blot analysis of lysates from wild type (WT) and CRYZ knockout (KO) HeLa cells, using [KO Validated] CRYZ Rabbit polyclonal antibody (STJ11100873) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 5s.](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/300x300/products/89807/358274/STJ11100873_1__03594.1713122620.jpg?c=1 "Western blot analysis of lysates from wild type (WT) and CRYZ knockout (KO) HeLa cells, using [KO Validated] CRYZ Rabbit polyclonal antibody (STJ11100873) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 5s.")
