Western blot analysis of various lysates using RAIDD/CRADD Rabbit mAb (STJ11102348) at 1:3000 dilution. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 30s.

Anti-CRADD antibody (100-199) [S8MR] (STJ11102348)

⇓ Datasheet ⇓ SDS
SPECIFICATIONS
ClonalityMonoclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
STJ11102348
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General Information

Short DescriptionRabbit monoclonal RAIDD (100-199) antibody for use in WB, IF, ICC and ELISA in human and rat samples. Datasheet included with dilution recommendations, and related reagents.
ApplicationsWB/IF/ICC/ELISA
HostRabbit
ReactivityHuman/Rat
NoteSTRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.

Product Properties

ClonalityMonoclonal
Clone IDS8MR
IsotypeIgG
ConjugationUnconjugated
ConcentrationLot specific
PurificationAffinity purification
Dilution RangeWB:1:500-1:1000
IF/ICC:1:50-1:200
ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements.
FormulationPBS with 0.02% Sodium Azide, 0.05% BSA, 50% Glycerol, pH 7.3.
Storage InstructionStore at-20ยฐC for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.

Target Information

Gene SymbolCRADD
Gene ID8738
Uniprot IDCRADD_HUMAN
Immunogen Region100-199
Immunogen SequenceGDRLTGIPSHILNSSPSDRQ INQLAQRLGPEWEPMVLSLG LSQTDIYRCKANHPHNVQSQ VVEAFIRWRQRFGKQATFQS LHNGLRAVEVDPSLLLHMLE
SpecificityA synthetic peptide corresponding to a sequence within amino acids 100-199 of human RAIDD/CRADD (NP_003796.1).

Additional Info

Tissue Specificity Constitutively expressed in most tissues, with particularly high expression in adult heart, testis, liver, skeletal muscle, fetal liver and kidney.
Function Adapter protein that associates with PIDD1 and the caspase CASP2 to form the PIDDosome, a complex that activates CASP2 and triggers apoptosis. Also recruits CASP2 to the TNFR-1 signaling complex through its interaction with RIPK1 and TRADD and may play a role in the tumor necrosis factor-mediated signaling pathway.
Protein Name Death Domain-Containing Protein Cradd
Caspase And Rip Adapter With Death Domain
Rip-Associated Protein With A Death Domain
Database Links Reactome: R-HSA-6803207
Cellular Localisation Cytoplasm
Nucleus
Alternative Antibody Names Anti-Death Domain-Containing Protein Cradd antibody
Anti-Caspase And Rip Adapter With Death Domain antibody
Anti-Rip-Associated Protein With A Death Domain antibody
Anti-CRADD antibody
Anti-RAIDD antibody

Information sourced from Uniprot.org

Citations

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