| Host: |
Rabbit |
| Applications: |
WB |
| Reactivity: |
Human/Mouse/Rat |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: |
Rabbit monoclonal antibody anti-CPSF73 (585-684) is suitable for use in Western Blot research applications. |
| Clonality: |
Monoclonal |
| Clone ID: |
S8MR |
| Conjugation: |
Unconjugated |
| Isotype: |
IgG |
| Formulation: |
PBS with 0.02% Sodium Azide, 0.05% BSA, 50% Glycerol, pH7.3. |
| Purification: |
Affinity purification |
| Dilution Range: |
WB 1:500-1:1000 |
| Storage Instruction: |
Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: |
CPSF3 |
| Gene ID: |
51692 |
| Uniprot ID: |
CPSF3_HUMAN |
| Immunogen Region: |
585-684 |
| Immunogen: |
A synthetic peptide corresponding to a sequence within amino acids 585-684 of human CPSF3 (Q9UKF6). |
| Immunogen Sequence: |
LEVQSNPKIRKGAVQKVSKK LEMHVYSKRLEIMLQDIFGE DCVSVKDDSILSVTVDGKTA NLNLETRTVECEEGSEDDES LREMVELAAQRLYEALTPVH |
| Post Translational Modifications | Sumoylated on Lys-462, Lys-465 and Lys-545, preferentially by SUMO3. |
| Function | Component of the cleavage and polyadenylation specificity factor (CPSF) complex that plays a key role in pre-mRNA 3'-end formation, recognizing the AAUAAA signal sequence and interacting with poly(A) polymerase and other factors to bring about cleavage and poly(A) addition. Has endonuclease activity, and functions as mRNA 3'-end-processing endonuclease. Also involved in the histone 3'-end pre-mRNA processing. U7 snRNP-dependent protein that induces both the 3'-endoribonucleolytic cleavage of histone pre-mRNAs and acts as a 5' to 3' exonuclease for degrading the subsequent downstream cleavage product (DCP) of mature histone mRNAs. Cleavage occurs after the 5'-ACCCA-3' sequence in the histone pre-mRNA leaving a 3'hydroxyl group on the upstream fragment containing the stem loop (SL) and 5' phosphate on the downstream cleavage product (DCP) starting with CU nucleotides. The U7-dependent 5' to 3' exonuclease activity is processive and degrades the DCP RNA substrate even after complete removal of the U7-binding site. Binds to the downstream cleavage product (DCP) of histone pre-mRNAs and the cleaved DCP RNA substrate in a U7 snRNP dependent manner. Required for entering/progressing through S-phase of the cell cycle. Required for the selective processing of microRNAs (miRNAs) during embryonic stem cell differentiation via its interaction with ISY1. Required for the biogenesis of all miRNAs from the pri-miR-17-92 primary transcript except miR-92a. Only required for the biogenesis of miR-290 and miR-96 from the pri-miR-290-295 and pri-miR-96-183 primary transcripts, respectively. |
| Protein Name | Cleavage And Polyadenylation Specificity Factor Subunit 3Cleavage And Polyadenylation Specificity Factor 73 Kda SubunitCpsf 73 Kda SubunitMrna 3'-End-Processing Endonuclease Cpsf-73 |
| Database Links | Reactome: R-HSA-159231Reactome: R-HSA-72187Reactome: R-HSA-72203Reactome: R-HSA-73856Reactome: R-HSA-77595 |
| Cellular Localisation | Nucleus |
| Alternative Antibody Names | Anti-Cleavage And Polyadenylation Specificity Factor Subunit 3 antibodyAnti-Cleavage And Polyadenylation Specificity Factor 73 Kda Subunit antibodyAnti-Cpsf 73 Kda Subunit antibodyAnti-Mrna 3'-End-Processing Endonuclease Cpsf-73 antibodyAnti-CPSF3 antibodyAnti-CPSF73 antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
