• Confocal imaging of paraffin-embedded Human liver using CPS1 Rabbit monoclonal antibody (STJ11102018, dilution 1:100) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) ( dilution 1:500) (Red). DAPI was used for nuclear staining (Blue). Objective: 40x. Perform high pressure antigen retrieval with 0. 01 M citrate buffer (pH 6. 0) prior to IF staining.
  • Confocal imaging of paraffin-embedded Rat liver using CPS1 Rabbit monoclonal antibody (STJ11102018, dilution 1:100) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) ( dilution 1:500) (Red). DAPI was used for nuclear staining (Blue). Objective: 40x. Perform high pressure antigen retrieval with 0. 01 M citrate buffer (pH 6. 0) prior to IF staining.
  • Immunohistochemistry analysis of CPS1 in paraffin-embedded human liver using CPS1 Rabbit monoclonal antibody (STJ11102018) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Western blot analysis of various lysates using CPS1 Rabbit monoclonal antibody (STJ11102018) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.

Anti-CPS1 antibody (100-200) [S8MR] (STJ11102018)

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STJ11102018

£146.50 - £639.50
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Host: Rabbit
Applications: WB/IHC/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit monoclonal antibody anti-CPS1 (100-200) is suitable for use in Western Blot, Immunohistochemistry and Immunofluorescence research applications.
Clonality: Monoclonal
Clone ID: S8MR
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 0.05% BSA, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IHC-P 1:50-1:200
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: CPS1
Gene ID: 1373
Uniprot ID: CPSM_HUMAN
Immunogen Region: 100-200
Immunogen: A synthetic peptide corresponding to a sequence within amino acids 100-200 of human CPS1 (NP_001866.2).
Immunogen Sequence: IIGNGGAPDTTALDELGLSK YLESNGIKVSGLLVLDYSKD YNHWLATKSLGQWLQEEKVP AIYGVDTRMLTKIIRDKGTM LGKIEFEGQPVDFVDPNKQN L
Tissue Specificity Primarily in the liver and small intestine.
Post Translational Modifications Undergoes proteolytic cleavage in the C-terminal region corresponding to the loss of approximately 12 AA residues from the C-terminus. Succinylated at Lys-287 and Lys-1291. Desuccinylated at Lys-1291 by SIRT5, leading to activation. Glutarylated. Glutarylation levels increase during fasting. Deglutarylated by SIRT5 at Lys-55, Lys-219, Lys-412, Lys-889, Lys-892, Lys-915, Lys-1360 and Lys-1486, leading to activation.
Function Involved in the urea cycle of ureotelic animals where the enzyme plays an important role in removing excess ammonia from the cell.
Protein Name Carbamoyl-Phosphate Synthase Ammonia - Mitochondrial
Carbamoyl-Phosphate Synthetase I
Cpsase I
Database Links Reactome: R-HSA-70635
Cellular Localisation Mitochondrion
Nucleus
Nucleolus
Cell Membrane
Peripheral Membrane Protein
Extracellular Side
Localizes To The Cell Surface Of Hepatocytes
Alternative Antibody Names Anti-Carbamoyl-Phosphate Synthase Ammonia - Mitochondrial antibody
Anti-Carbamoyl-Phosphate Synthetase I antibody
Anti-Cpsase I antibody
Anti-CPS1 antibody

Information sourced from Uniprot.org

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