Anti-CYP51A1 antibody (210-290 aa) (STJ190290)
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
ImmunogenSynthesized peptide derived from the human protein at the amino acid range 210-290
General Information
| Short Description | Rabbit polyclonal anti-Lanosterol 14-alpha demethylase (210-290 aa) for use in WB and ELISA in Human, Rat and Mouse samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB/ELISA |
| Host | Rabbit |
| Reactivity | Human/Rat/Mouse |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | 1 mg/mL |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Dilution Range | WB 1:500-2000ELISA 1:5000-20000 |
| Formulation | Liquid in PBS containing 50% Glycerol and 0.02% Sodium Azide. |
| Storage Instruction | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | CYP51A1 |
| Gene ID | 1595 |
| Uniprot ID | CP51A_HUMAN |
| Immunogen | Synthesized peptide derived from the human protein at the amino acid range 210-290 |
| Immunogen Region | 210-290 aa |
| Specificity | CP51A Polyclonal Antibody detects endogenous levels of protein. |
Additional Info
| Post Translational Modifications | Ubiquitinated by MARCHF6, leading to proteasomal degradation. |
| Function | Sterol 14alpha-demethylase that plays a critical role in the cholesterol biosynthesis pathway, being cholesterol the major sterol component in mammalian membranes as well as a precursor for bile acid and steroid hormone synthesis. Cytochrome P450 monooxygenase that catalyzes the three-step oxidative removal of the 14alpha-methyl group (C-32) of sterols such as lanosterol (lanosta-8,24-dien-3beta-ol) and 24,25-dihydrolanosterol (DHL) in the form of formate, and converts the sterols to 4,4-dimethyl-5alpha-cholesta-8,14,24-trien-3beta-ol and 4,4-dimethyl-8,14-cholestadien-3beta-ol, respectively, which are intermediates of cholesterol biosynthesis. Can also demethylate substrates not intrinsic to mammals, such as eburicol (24-methylene-24,25-dihydrolanosterol), but at a lower rate than DHL. |
| Protein Name | Lanosterol 14-Alpha DemethylaseLdmCypliCytochrome P450 51a1Cyp51a1Cytochrome P450-14dmCytochrome P45014dmCytochrome P450liSterol 14-Alpha Demethylase |
| Database Links | Reactome: R-HSA-191273Reactome: R-HSA-211976Reactome: R-HSA-2426168Reactome: R-HSA-9619665 |
| Cellular Localisation | Endoplasmic Reticulum MembraneSingle-Pass Membrane ProteinMicrosome Membrane |
| Alternative Antibody Names | Anti-Lanosterol 14-Alpha Demethylase antibodyAnti-Ldm antibodyAnti-Cypli antibodyAnti-Cytochrome P450 51a1 antibodyAnti-Cyp51a1 antibodyAnti-Cytochrome P450-14dm antibodyAnti-Cytochrome P45014dm antibodyAnti-Cytochrome P450li antibodyAnti-Sterol 14-Alpha Demethylase antibodyAnti-CYP51A1 antibodyAnti-CYP51 antibody |
Information sourced from Uniprot.org