Anti-CP110 antibody (N-Term) (STJ72724)
SPECIFICATIONS
ClonalityPolyclonal
HostGoat
ConjugationUnconjugated
IsotypeIgG
General Information
| Short Description | Goat polyclonal antibody anti-CP110 (N-Term) is suitable for use in ELISA and Western Blot research applications. |
| Applications | Pep-ELISA/WB |
| Host | Goat |
| Reactivity | Human |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | 0.5 mg/mL |
| Purification | Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
| Dilution Range | Peptide ELISA: antibody detection limit dilution 1:32000.WB: Approx 110kDa band observed in Human Testis lysates (calculated MW of 113kDa according to NP_001185951.1). Recommended concentration: 0.3-1ยตg/ml.Additional bands of unknown identity we |
| Formulation | 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. NA |
| Storage Instruction | Store at-20ยฐC on receipt and minimise freeze-thaw cycles. |
Target Information
| Gene Symbol | CCP110 |
| Gene ID | 9738 |
| Uniprot ID | CP110_HUMAN |
| Accession Number | NP_001185951.1; NP_055526.3 |
| Immunogen Region | N-Term |
| Immunogen Sequence | CEKSLARIQEAS |
| Specificity | This antibody is expected to recognize both reported isoforms (NP_001185951.1; NP_055526.3). |
Additional Info
| Post Translational Modifications | Phosphorylated by CDKs. Ubiquitinated by the SCF(CCNF) during G2 phase, leading to its degradation by the proteasome and preventing centrosome reduplication. Deubiquitinated by USP33 in S and G2/M phase, leading to stabilize CCP110 during the period which centrioles duplicate and elongate. Ubiquitinated by the EDVP complex, leading to its degradation. |
| Function | Necessary for centrosome duplication at different stages of procentriole formation. Acts as a key negative regulator of ciliogenesis in collaboration with CEP97 by capping the mother centriole thereby preventing cilia formation. Also involved in promoting ciliogenesis. May play a role in the assembly of the mother centriole subdistal appendages (SDA) thereby effecting the fusion of recycling endosomes to basal bodies during cilia formation. Required for correct spindle formation and has a role in regulating cytokinesis and genome stability via cooperation with CALM1 and CETN2. |
| Protein Name | Centriolar Coiled-Coil Protein Of 110 KdaCentrosomal Protein Of 110 KdaCp110Cep110 |
| Database Links | Reactome: R-HSA-2565942Reactome: R-HSA-380259Reactome: R-HSA-380270Reactome: R-HSA-380284Reactome: R-HSA-380320Reactome: R-HSA-5620912Reactome: R-HSA-5689880Reactome: R-HSA-8854518Reactome: R-HSA-9013424 |
| Cellular Localisation | CytoplasmCytoskeletonMicrotubule Organizing CenterCentrosomeCentrioleCilium Basal BodyRecruited Early And Then Associates With The Growing Distal TipsRecruited To The Mother Centriole By Kif24Removed From Centrioles By Ttbk2Leading To Initiation Of Ciliogenesis And Localizes Only To The Daughter Centriole In Ciliated CellsIn Cytotoxic T Lymphocytes Remains Associated With The Mother Centriole During Docking Of The Centrosome At The Immunological Synapse Upon Target ContactRecruited At The Distal End Of The Mother Centriole By Mphosph9 |
| Alternative Antibody Names | Anti-Centriolar Coiled-Coil Protein Of 110 Kda antibodyAnti-Centrosomal Protein Of 110 Kda antibodyAnti-Cp110 antibodyAnti-Cep110 antibodyAnti-CCP110 antibodyAnti-CEP110 antibodyAnti-CP110 antibodyAnti-KIAA0419 antibody |
Information sourced from Uniprot.org