• Immunofluorescence analysis of L929 cells using COX15 Rabbit polyclonal antibody (STJ116869) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
  • Immunohistochemistry analysis of COX15 in paraffin-embedded mouse heart using COX15 Rabbit polyclonal antibody (STJ116869) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of COX15 in paraffin-embedded human thyroid cancer using COX15 Rabbit polyclonal antibody (STJ116869) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of COX15 in paraffin-embedded rat spleen using COX15 Rabbit polyclonal antibody (STJ116869) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Western blot analysis of various lysates using COX15 Rabbit polyclonal antibody (STJ116869) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 5s.

Anti-COX15 antibody (150-250) (STJ116869)

SKU:
STJ116869

£112.50 - £524.50
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Host: Rabbit
Applications: WB/IHC-P/IF/ICC/ELISA
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH 7.3.
Purification: Affinity purification
Concentration: Lot specific
Dilution Range: WB:1:500-1:2000
IHC-P:1:50-1:100
IF/ICC:1:50-1:100
ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements.
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: COX15
Gene ID: 1355
Uniprot ID: COX15_HUMAN
Immunogen Region: 150-250
Specificity: A synthetic peptide corresponding to a sequence within amino acids 150-250 of human COX15 (NP_510870.1).
Immunogen Sequence: YSHRMWGRLVGLVYILPAAY FWRKGWLSRGMKGRVLALCG LVCFQGLLGWYMVKSGLEEK SDSHDIPRVSQYRLAAHLGS ALVLYCASLWTSLSLLLPPH K
Tissue Specificity Predominantly found in tissues characterized by high rates of oxidative phosphorylation (OxPhos), including muscle, heart, and brain.
Function Catalyzes the second reaction in the biosynthesis of heme A, a prosthetic group of mitochondrial cytochrome c oxidase (CcO). Heme A is synthesized from heme B by two sequential enzymatic reactions catalyzed by heme O synthase (HOS) and heme A synthase (HAS). HAS catalyzes the conversion of heme O to heme A by two successive hydroxylations of the methyl group at C8, in a reaction that involves matrix ferredoxin and ferredoxin reductase. The first hydroxylation forms heme I, the second hydroxylation results in an unstable dihydroxymethyl group, which spontaneously dehydrates, resulting in the formyl group of heme A.
Protein Name Heme A Synthase Cox15
Has
Cytochrome C Oxidase Assembly Protein Cox15 Homolog
Database Links Reactome: R-HSA-189451
Reactome: R-HSA-9864848
Cellular Localisation Mitochondrion Inner Membrane
Multi-Pass Membrane Protein
Alternative Antibody Names Anti-Heme A Synthase Cox15 antibody
Anti-Has antibody
Anti-Cytochrome C Oxidase Assembly Protein Cox15 Homolog antibody
Anti-COX15 antibody

Information sourced from Uniprot.org

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