• Immunofluorescence analysis of L929 cells using COPZ1 Rabbit polyclonal antibody (STJ114041) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of U2OS cells using COPZ1 Rabbit polyclonal antibody (STJ114041) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
  • Western blot analysis of various lysates using COPZ1 Rabbit polyclonal antibody (STJ114041) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.

Anti-COPZ1 antibody (1-177) (STJ114041)

SKU:
STJ114041

£112.50 - £522.50
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Host: Rabbit
Applications: WB/IF
Reactivity: Human/Mouse
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-COPZ1 (1-177) is suitable for use in Western Blot and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: COPZ1
Gene ID: 22818
Uniprot ID: COPZ1_HUMAN
Immunogen Region: 1-177
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-177 of human COPZ1 (NP_057141.1).
Immunogen Sequence: MEALILEPSLYTVKAILILD NDGDRLFAKYYDDTYPSVKE QKAFEKNIFNKTHRTDSEIA LLEGLTVVYKSSIDLYFYVI GSSYENELMLMAVLNCLFDS LSQMLRKNVEKRALLENMEG LFLAVDEIVDGGVILESDPQ QVVHRVALRGEDVPLTEQTV SQVLQSAKEQIKWSLLR
Function The coatomer is a cytosolic protein complex that binds to dilysine motifs and reversibly associates with Golgi non-clathrin-coated vesicles, which further mediate biosynthetic protein transport from the ER, via the Golgi up to the trans Golgi network. Coatomer complex is required for budding from Golgi membranes, and is essential for the retrograde Golgi-to-ER transport of dilysine-tagged proteins. The zeta subunit may be involved in regulating the coat assembly and, hence, the rate of biosynthetic protein transport due to its association-dissociation properties with the coatomer complex.
Protein Name Coatomer Subunit Zeta-1
Zeta-1-Coat Protein
Zeta-1 Cop
Database Links Reactome: R-HSA-6807878
Reactome: R-HSA-6811434
Cellular Localisation Cytoplasm
Golgi Apparatus Membrane
Peripheral Membrane Protein
Cytoplasmic Side
Cytoplasmic Vesicle
Copi-Coated Vesicle Membrane
The Coatomer Is Cytoplasmic Or Polymerized On The Cytoplasmic Side Of The Golgi
As Well As On The Vesicles/Buds Originating From It
Alternative Antibody Names Anti-Coatomer Subunit Zeta-1 antibody
Anti-Zeta-1-Coat Protein antibody
Anti-Zeta-1 Cop antibody
Anti-COPZ1 antibody
Anti-COPZ antibody
Anti-CGI-120 antibody
Anti-HSPC181 antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance