| Host: | Goat |
| Applications: | Pep-ELISA/WB/FC |
| Reactivity: | Human/Dog |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description : | Goat polyclonal antibody anti-COMT (N-Term) is suitable for use in ELISA, Western Blot and Flow Cytometry research applications. |
| Clonality : | Polyclonal |
| Conjugation: | Unconjugated |
| Isotype: | IgG |
| Formulation: | 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. NA |
| Purification: | Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
| Concentration: | 0.5 mg/mL |
| Dilution Range: | Peptide ELISA: antibody detection limit dilution 1:4000.WB: Approx 25kDa band observed in Human Liver and Ovary lysates and in MCF7 cell lysates, and approx. 25kDa and 30kDa in U251 cell lysate (calculated MW of 30.0kDa according to NP_000745.1 |
| Storage Instruction: | Store at-20°C on receipt and minimise freeze-thaw cycles. |
| Gene Symbol: | COMT |
| Gene ID: | 1312 |
| Uniprot ID: | COMT_HUMAN |
| Immunogen Region: | N-Term |
| Accession Number: | NP_000745.1; NP_001128633.1; NP_001128634.1; NP_009294.1 |
| Specificity: | This antibody is expected to recognise both reported isoforms S-COMT NP_009294.1 and MB-COMT NP_000745.1; NP_001128633.1; NP_001128634.. |
| Immunogen Sequence: | GDTKEQRILNHVLQC |
| Post Translational Modifications | The N-terminus is blocked. |
| Function | Catalyzes the O-methylation, and thereby the inactivation, of catecholamine neurotransmitters and catechol hormones. Also shortens the biological half-lives of certain neuroactive drugs, like L-DOPA, alpha-methyl DOPA and isoproterenol. |
| Protein Name | Catechol O-Methyltransferase |
| Database Links | Reactome: R-HSA-156581Reactome: R-HSA-379397Reactome: R-HSA-379398Reactome: R-HSA-9679191 |
| Cellular Localisation | Isoform Soluble: CytoplasmIsoform Membrane-Bound: Cell MembraneSingle-Pass Type Ii Membrane ProteinExtracellular Side |
| Alternative Antibody Names | Anti-Catechol O-Methyltransferase antibodyAnti-COMT antibody |
Information sourced from Uniprot.org