Western blot analysis of various lysates using Collagen VI/COL6A1 Rabbit pAb (STJ111622) at 1:1000 dilution. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 1s.

Anti-COL6A1 antibody (19-250) (STJ111622)

⇓ Datasheet ⇓ SDS
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
STJ111622
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General Information

Short DescriptionRabbit polyclonal COL6A1 (19-250) antibody for use in WB and ELISA in human and mouse samples. Datasheet included with dilution recommendations, and related reagents.
ApplicationsWB/ELISA
HostRabbit
ReactivityHuman/Mouse
NoteSTRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.

Product Properties

ClonalityPolyclonal
IsotypeIgG
ConjugationUnconjugated
ConcentrationLot specific
PurificationAffinity purification
Dilution RangeWB:1:500-1:2000
ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements.
FormulationPBS with 0.02% Sodium Azide, 50% Glycerol, pH 7.3.
Storage InstructionStore at-20ยฐC for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.

Target Information

Gene SymbolCOL6A1
Gene ID1291
Uniprot IDCO6A1_HUMAN
Immunogen Region19-250
Immunogen SequenceAQDEPETPRAVAFQDCPVDL FFVLDTSESVALRLKPYGAL VDKVKSFTKRFIDNLRDRYY RCDRNLVWNAGALHYSDEVE IIQGLTRMPGGRDALKSSVD AVKYFGKGTYTDCAIKKGLE QLLVGGSHLKENKYLIVVTD GHPLEGYKEPCGGLEDAVNE AKHLGVKVFSVAITPDHLEP RLSIIATDHTYRRNFTAADW GQSRDAEEAISQTIDTIVDM IKNNVEQVCCSF
SpecificityRecombinant fusion protein containing a sequence corresponding to amino acids 19-250 of human Collagen VI/Collagen VI/COL6A1 (NP_001839.2).

Additional Info

Post Translational Modifications Prolines at the third position of the tripeptide repeating unit (G-X-Y) are hydroxylated in some or all of the chains.
Function Collagen VI acts as a cell-binding protein.
Protein Name Collagen Alpha-1(Vi Chain
Database Links Reactome: R-HSA-1442490
Reactome: R-HSA-1650814
Reactome: R-HSA-186797
Reactome: R-HSA-2022090
Reactome: R-HSA-216083
Reactome: R-HSA-3000178
Reactome: R-HSA-419037
Reactome: R-HSA-8948216
Cellular Localisation Secreted
Extracellular Space
Extracellular Matrix
Alternative Antibody Names Anti-Collagen Alpha-1(Vi Chain antibody
Anti-COL6A1 antibody

Information sourced from Uniprot.org

Citations

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