• Western blot analysis of A-204, using COL11A1 rabbit polyclonal antibody (STJ118005) at 1:2000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.
  • Immunohistochemistry analysis of paraffin-embedded rat heart using COL11A1 antibody (STJ118005) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of paraffin-embedded mouse heart using COL11A1 antibody (STJ118005) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.

Anti-COL11A1 antibody (260-430) (STJ118005)

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STJ118005

€140.63 - €653.13
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Host: Rabbit
Applications: WB/IHC
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-COL11A1 (260-430) is suitable for use in Western Blot and Immunohistochemistry research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:1000-1:5000
IHC-P 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: COL11A1
Gene ID: 1301
Uniprot ID: COBA1_HUMAN
Immunogen Region: 260-430
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 260-430 of human COL11A1 (NP_542196.2).
Immunogen Sequence: EKKKSNFKKKMRTVATKSKE KSKKFTPPKSEKFSSKKKKS YQASAKAKLGVKANIVDDFQ EYNYGTMESYQTEAPRHVSG TNEPNPVEEIFTEEYLTGED YDSQRKNSEDTLYENKEIDG RDSDLLVDGDLGEYDFYEYK EYEDKPTSPPNEEFGPGVPA ETDITETSING
Tissue Specificity Cartilage, placenta and some tumor or virally transformed cell lines. Isoforms using exon IIA or IIB are found in the cartilage while isoforms using only exon IIB are found in the tendon.
Post Translational Modifications Prolines at the third position of the tripeptide repeating unit (G-X-Y) are hydroxylated in some or all of the chains. N-glycosylated.
Function May play an important role in fibrillogenesis by controlling lateral growth of collagen II fibrils.
Protein Name Collagen Alpha-1(Xi Chain
Database Links Reactome: R-HSA-1442490
Reactome: R-HSA-1650814
Reactome: R-HSA-2022090
Reactome: R-HSA-3000171
Reactome: R-HSA-8874081
Reactome: R-HSA-8948216
Cellular Localisation Secreted
Extracellular Space
Extracellular Matrix
Alternative Antibody Names Anti-Collagen Alpha-1(Xi Chain antibody
Anti-COL11A1 antibody
Anti-COLL6 antibody

Information sourced from Uniprot.org

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