• Western blot analysis of extracts of Rat lung, using Collagen X/COL10A1 antibody (STJ11100547) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.
  • Immunohistochemistry of paraffin-embedded human colon using Collagen X/COL10A1 rabbit polyclonal antibody (STJ11100547) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.

Anti-COL10A1 antibody (521-680) (STJ11100547)

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STJ11100547

£169.50 - £449.50
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Host: Rabbit
Applications: WB/IHC
Reactivity: Human/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-COL10A1 (521-680) is suitable for use in Western Blot and Immunohistochemistry research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:100-1:500
IHC-P 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: COL10A1
Gene ID: 1300
Uniprot ID: COAA1_HUMAN
Immunogen Region: 521-680
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 521-680 of human Collagen X/COL10A1 (NP_000484.2).
Immunogen Sequence: MPEGFIKAGQRPSLSGTPLV SANQGVTGMPVSAFTVILSK AYPAIGTPIPFDKILYNRQQ HYDPRTGIFTCQIPGIYYFS YHVHVKGTHVWVGLYKNGTP VMYTYDEYTKGYLDQASGSA IIDLTENDQVWLQLPNAESN GLYSSEYVHSSFSGFLVAPM
Post Translational Modifications Prolines at the third position of the tripeptide repeating unit (G-X-Y) are hydroxylated in some or all of the chains.
Function Type X collagen is a product of hypertrophic chondrocytes and has been localized to presumptive mineralization zones of hyaline cartilage.
Protein Name Collagen Alpha-1(X Chain
Database Links Reactome: R-HSA-1442490
Reactome: R-HSA-1650814
Reactome: R-HSA-2022090
Reactome: R-HSA-216083
Reactome: R-HSA-3000171
Reactome: R-HSA-8948216
Cellular Localisation Secreted
Extracellular Space
Extracellular Matrix
Alternative Antibody Names Anti-Collagen Alpha-1(X Chain antibody
Anti-COL10A1 antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance