• Western blot analysis of extracts of various cell lines, using CoA synthase (COASY) antibody (STJ11102722) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.

Anti-CoA synthase antibody (465-564) [S2MR] (STJ11102722)

SKU:
STJ11102722

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Host: Rabbit
Applications: WB
Reactivity: Human
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit monoclonal antibody anti-CoA synthase (COASY ) (465-564) is suitable for use in Western Blot research applications.
Clonality: Monoclonal
Clone ID: S2MR
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 0.05% BSA, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:1000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: COASY
Gene ID: 80347
Uniprot ID: COASY_HUMAN
Immunogen Region: 465-564
Immunogen: A synthetic peptide corresponding to a sequence within amino acids 465-564 of human CoA synthase (COASY ) (Q13057).
Immunogen Sequence: RVCVIDAAVLLEAGWQNLVH EVWTAVIPETEAVRRIVERD GLSEAAAQSRLQSQMSGQQL VEQSHVVLSTLWEPHITQRQ VEKAWALLQKRIPKTHQALD
Tissue Specificity Expressed in all tissues examined including brain, heart, skeletal muscle, colon, thymus, spleen, kidney, liver, small intestine, placenta, lung and peripheral blood leukocyte. Lowest expression in peripheral blood leukocytes and highest in kidney and liver. Isoform 2 is expressed mainly in the brain.
Function Bifunctional enzyme that catalyzes the fourth and fifth sequential steps of CoA biosynthetic pathway. The fourth reaction is catalyzed by the phosphopantetheine adenylyltransferase, coded by the coaD domain.the fifth reaction is catalyzed by the dephospho-CoA kinase, coded by the coaE domain. May act as a point of CoA biosynthesis regulation.
Protein Name Bifunctional Coenzyme A Synthase
Coa Synthase
Nbp
Pov-2 Includes - Phosphopantetheine Adenylyltransferase
Dephospho-Coa Pyrophosphorylase
Pantetheine-Phosphate Adenylyltransferase
Ppat - Dephospho-Coa Kinase
Dpck
Dephosphocoenzyme A Kinase
Dpcoak
Database Links Reactome: R-HSA-196783
Cellular Localisation Cytoplasm
Mitochondrion Matrix
The Protein Is Mainly Present In The Mitochondrial Matrix
Probably Anchored To The Inner Mitochondrial Membrane
But Is Also Present In Cell Lysate
Alternative Antibody Names Anti-Bifunctional Coenzyme A Synthase antibody
Anti-Coa Synthase antibody
Anti-Nbp antibody
Anti-Pov-2 Includes - Phosphopantetheine Adenylyltransferase antibody
Anti-Dephospho-Coa Pyrophosphorylase antibody
Anti-Pantetheine-Phosphate Adenylyltransferase antibody
Anti-Ppat - Dephospho-Coa Kinase antibody
Anti-Dpck antibody
Anti-Dephosphocoenzyme A Kinase antibody
Anti-Dpcoak antibody
Anti-COASY antibody
Anti-PSEC0106 antibody

Information sourced from Uniprot.org

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