• Immunohistochemistry analysis of CLN8 in paraffin-embedded human brain using CLN8 Rabbit polyclonal antibody (STJ119197) at dilution of 1:300 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Western blot analysis of various lysates using CLN8 Rabbit polyclonal antibody (STJ119197) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 90s.

Anti-CLN8 antibody (200-286) (STJ119197)

SKU:
STJ119197

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Host: Rabbit
Applications: WB/IHC
Reactivity: Human
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-CLN8 (200-286) is suitable for use in Western Blot and Immunohistochemistry research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IHC-P 1:100-1:500
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: CLN8
Gene ID: 2055
Uniprot ID: CLN8_HUMAN
Immunogen Region: 200-286
Immunogen: A synthetic peptide corresponding to a sequence within amino acids 200-286 of human CLN8 (NP_061764.2).
Immunogen Sequence: MFHCRMVLTYHMWWVCFWHW DGLVSSLYLPHLTLFLVGLA LLTLIINPYWTHKKTQQLLN PVDWNFAQPEAKSRPEGNGQ LLRKKRP
Post Translational Modifications Does not seem to be N-glycosylated.
Function Could play a role in cell proliferation during neuronal differentiation and in protection against cell death.
Protein Name Protein Cln8
Cellular Localisation Endoplasmic Reticulum Membrane
Multi-Pass Membrane Protein
Endoplasmic Reticulum-Golgi Intermediate Compartment Membrane
Endoplasmic Reticulum
Alternative Antibody Names Anti-Protein Cln8 antibody
Anti-CLN8 antibody
Anti-C8orf61 antibody

Information sourced from Uniprot.org

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