Anti-Cleaved-NOTCH3-Asp1628 antibody (1628-1629) (STJA0005904)

SKU:
STJA0005904

Current Stock:
Host: Rabbit
Applications: WB/ELISA
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-Cleaved-Neurogenic locus notch homolog protein 3-Asp1628 (1628-1629) is suitable for use in Western Blot and ELISA research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide.
Purification: The antibody was affinity-purified from rabbit serum by affinity-chromatography using specific immunogen.
Concentration: 1 mg/mL
Dilution Range: WB 1:1000-2000
ELISA 1:5000-20000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: NOTCH3
Gene ID: 4854
Uniprot ID: NOTC3_HUMAN
Immunogen Region: 1628-1629
Specificity: This antibody detects endogenous levels of Human, Mouse, Rat NOTCH3 (Cleaved-Asp1628, protein was cleaved amino acid sequence between 1628-1629 )
Immunogen: Synthesized peptide derived from human NOTCH3 (Cleaved-Asp1628)
Post Translational Modifications Synthesized in the endoplasmic reticulum as an inactive form which is proteolytically cleaved by a furin-like convertase in the trans-Golgi network before it reaches the plasma membrane to yield an active, ligand-accessible form. Cleavage results in a C-terminal fragment N(TM) and a N-terminal fragment N(EC). Following ligand binding, it is cleaved by TNF-alpha converting enzyme (TACE) to yield a membrane-associated intermediate fragment called notch extracellular truncation (NEXT). This fragment is then cleaved by presenilin dependent gamma-secretase to release a notch-derived peptide containing the intracellular domain (NICD) from the membrane. Phosphorylated. Hydroxylated by HIF1AN.
Function Functions as a receptor for membrane-bound ligands Jagged1, Jagged2 and Delta1 to regulate cell-fate determination. Upon ligand activation through the released notch intracellular domain (NICD) it forms a transcriptional activator complex with RBPJ/RBPSUH and activates genes of the enhancer of split locus. Affects the implementation of differentiation, proliferation and apoptotic programs.
Protein Name Neurogenic Locus Notch Homolog Protein 3
Notch 3 Cleaved Into - Notch 3 Extracellular Truncation - Notch 3 Intracellular Domain
Database Links Reactome: R-HSA-1912399
Reactome: R-HSA-1912408
Reactome: R-HSA-1912420
Reactome: R-HSA-350054
Reactome: R-HSA-5083630
Reactome: R-HSA-9013507
Reactome: R-HSA-9013508
Reactome: R-HSA-9017802
Cellular Localisation Cell Membrane
Single-Pass Type I Membrane Protein
Notch 3 Intracellular Domain: Nucleus
Following Proteolytical Processing Nicd Is Translocated To The Nucleus
Alternative Antibody Names Anti-Neurogenic Locus Notch Homolog Protein 3 antibody
Anti-Notch 3 Cleaved Into - Notch 3 Extracellular Truncation - Notch 3 Intracellular Domain antibody
Anti-NOTCH3 antibody

Information sourced from Uniprot.org

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