• Western blot analysis of lysates from A549, 293, and HeLa cells, treated with etoposide 25uM 24h, using MMP3 (Cleaved-Phe100) Antibody. The lane on the right is blocked with the synthesized peptide.
  • Western blot analysis of various cells using Cleaved-MMP-3 (F100) Polyclonal Antibody

Anti-Cleaved-MMP3-F100 antibody (81-130 aa) (STJ90065)

SKU:
STJ90065

£46.00 - £226.00
Current Stock:
Host: Rabbit
Applications: WB/ELISA
Reactivity: Human/Rat/Mouse
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-Cleaved-Stromelysin-1-F100 (81-130 aa) is suitable for use in Western Blot and ELISA research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide.
Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Concentration: 1 mg/mL
Dilution Range: WB 1:500-1:2000
ELISA 1:20000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: MMP3
Gene ID: 4314
Uniprot ID: MMP3_HUMAN
Immunogen Region: 81-130 aa
Specificity: Cleaved-MMP-3 (F100) Polyclonal Antibody detects endogenous levels of fragment of activated MMP-3 protein resulting from cleavage adjacent to F100.
Immunogen: The antiserum was produced against synthesized peptide derived from the human MMP3 at the amino acid range 81-130
Function Metalloproteinase with a rather broad substrate specificity that can degrade fibronectin, laminin, gelatins of type I, III, IV, and V.collagens III, IV, X, and IX, and cartilage proteoglycans. Activates different molecules including growth factors, plasminogen or other matrix metalloproteinases such as MMP9. Once released into the extracellular matrix (ECM), the inactive pro-enzyme is activated by the plasmin cascade signaling pathway. Acts also intracellularly. For example, in dopaminergic neurons, gets activated by the serine protease HTRA2 upon stress and plays a pivotal role in DA neuronal degeneration by mediating microglial activation and alpha-synuclein/SNCA cleavage. In addition, plays a role in immune response and possesses antiviral activity against various viruses such as vesicular stomatitis virus, influenza A virus (H1N1) and human herpes virus 1. Mechanistically, translocates from the cytoplasm into the cell nucleus upon virus infection to influence NF-kappa-B activities.
Protein Name Stromelysin-1
Sl-1
Matrix Metalloproteinase-3
Mmp-3
Transin-1
Database Links Reactome: R-HSA-1442490
Reactome: R-HSA-1474228
Reactome: R-HSA-1592389
Reactome: R-HSA-2022090
Reactome: R-HSA-2179392
Reactome: R-HSA-6785807
Reactome: R-HSA-9009391
Cellular Localisation Secreted
Extracellular Space
Extracellular Matrix
Nucleus
Cytoplasm
Alternative Antibody Names Anti-Stromelysin-1 antibody
Anti-Sl-1 antibody
Anti-Matrix Metalloproteinase-3 antibody
Anti-Mmp-3 antibody
Anti-Transin-1 antibody
Anti-MMP3 antibody
Anti-STMY1 antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance