• Immunofluorescence analysis of MCF7 cells using CLDN3 antibody (STJ23159) at dilution of 1:100. Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of Mouse intestin using CLDN3 antibody (STJ23159) at dilution of 1:100. Blue: DAPI for nuclear staining.
  • Western blot analysis of extracts of various cell lines, using CLDN3 antibody (STJ23159) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.

Anti-CLDN3 antibody (141-220) (STJ23159)

SKU:
STJ23159

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Host: Rabbit
Applications: WB/IF
Reactivity: Human/Mouse
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-CLDN3 (141-220) is suitable for use in Western Blot and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.05% Proclin300, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:1000
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: CLDN3
Gene ID: 1365
Uniprot ID: CLD3_HUMAN
Immunogen Region: 141-220
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 141-220 of human CLDN3 (NP_001297.1).
Immunogen Sequence: TIIRDFYNPVVPEAQKREMG AGLYVGWAAAALQLLGGALL CCSCPPREKKYTATKVVYSA PRSTGPGASLGTGYDRKDYV
Function Plays a major role in tight junction-specific obliteration of the intercellular space, through calcium-independent cell-adhesion activity.
Protein Name Claudin-3
Clostridium Perfringens Enterotoxin Receptor 2
Cpe-R 2
Cpe-Receptor 2
Rat Ventral Prostate.1 Protein Homolog
Hrvp1
Database Links Reactome: R-HSA-420029
Cellular Localisation Cell Junction
Tight Junction
Cell Membrane
Multi-Pass Membrane Protein
Alternative Antibody Names Anti-Claudin-3 antibody
Anti-Clostridium Perfringens Enterotoxin Receptor 2 antibody
Anti-Cpe-R 2 antibody
Anti-Cpe-Receptor 2 antibody
Anti-Rat Ventral Prostate.1 Protein Homolog antibody
Anti-Hrvp1 antibody
Anti-CLDN3 antibody
Anti-C7orf1 antibody
Anti-CPETR2 antibody

Information sourced from Uniprot.org

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