Anti-CISH antibody (17-130) (STJ116738)
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
General Information
| Short Description | Rabbit polyclonal CISH (17-130) antibody for use in WB, IF, ICC and ELISA in human, mouse and rat samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB/IF/ICC/ELISA |
| Host | Rabbit |
| Reactivity | Human/Mouse/Rat |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | Lot specific |
| Purification | Affinity purification |
| Dilution Range | WB:1:1000-1:5000IF/ICC:1:50-1:200ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Formulation | PBS with 0.05% Proclin300, 50% Glycerol, pH 7.3. |
| Storage Instruction | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | CISH |
| Gene ID | 1154 |
| Uniprot ID | CISH_HUMAN |
| Immunogen Region | 17-130 |
| Immunogen Sequence | TGQRPLWAPSLELPKPVMQP LPAGAFLEEVAEGTPAQTES EPKVLDPEEDLLCIAKTFSY LRESGWYWGSITASEARQHL QKMPEGTFLVRDSTHPSYLF TLSVKTTRGPTNVR |
| Specificity | Recombinant fusion protein containing a sequence corresponding to amino acids 17-130 of human CISH (NP_659508.1). |
Additional Info
| Tissue Specificity | Expressed in various epithelial tissues. Abundantly expressed in liver and kidney, and to a lesser extent in lung. The tissue distribution of isoforms 1 and 1B is distinct. |
| Post Translational Modifications | Association with EPOR may target the protein for proteolysis by the ubiquitin-dependent proteasome pathway. CIS is mainly monubiquitinated (37 kDa form) but may also exist in a polyubiquitinated form (45 kDa). |
| Function | SOCS family proteins form part of a classical negative feedback system that regulates cytokine signal transduction. CIS is involved in the negative regulation of cytokines that signal through the JAK-STAT5 pathway such as erythropoietin, prolactin and interleukin 3 (IL3) receptor. Inhibits STAT5 trans-activation by suppressing its tyrosine phosphorylation. May be a substrate-recognition component of a SCF-like ECS (Elongin BC-CUL2/5-SOCS-box protein) E3 ubiquitin-protein ligase complex which mediates the ubiquitination and subsequent proteasomal degradation of target proteins. |
| Protein Name | Cytokine-Inducible Sh2-Containing ProteinCisCis-1Protein G18Suppressor Of Cytokine SignalingSocs |
| Database Links | Reactome: R-HSA-1266695Reactome: R-HSA-8951664Reactome: R-HSA-982772 |
| Cellular Localisation | |
| Alternative Antibody Names | Anti-Cytokine-Inducible Sh2-Containing Protein antibodyAnti-Cis antibodyAnti-Cis-1 antibodyAnti-Protein G18 antibodyAnti-Suppressor Of Cytokine Signaling antibodyAnti-Socs antibodyAnti-CISH antibodyAnti-G18 antibody |
Information sourced from Uniprot.org