• Confocal imaging of paraffin-embedded Human breast cancer tissue using CIRBP Rabbit monoclonal antibody (STJ11103019, dilution 1:100) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) ( dilution 1:500) (Red). DAPI was used for nuclear staining (Blue). Objective: 40x. Perform high pressure antigen retrieval with 0. 01 M citrate buffer (pH 6. 0) prior to IF staining.
  • Immunohistochemistry analysis of CIRBP in paraffin-embedded human thyroid tissue using CIRBP Rabbit monoclonal antibody (STJ11103019) at a dilution of 1:1000 (40x lens). High pressure antigen retrieval was performed with 0. 01 M citrate buffer (pH 6. 0) prior to immunohistochemistry staining.
  • Immunohistochemistry analysis of CIRBP in paraffin-embedded human lung tissue using CIRBP Rabbit monoclonal antibody (STJ11103019) at a dilution of 1:1000 (40x lens). High pressure antigen retrieval was performed with 0. 01 M citrate buffer (pH 6. 0) prior to immunohistochemistry staining.
  • Immunohistochemistry analysis of CIRBP in paraffin-embedded human pancreas tissue using CIRBP Rabbit monoclonal antibody (STJ11103019) at a dilution of 1:1000 (40x lens). High pressure antigen retrieval was performed with 0. 01 M citrate buffer (pH 6. 0) prior to immunohistochemistry staining.
  • Western blot analysis of various lysates using CIRBP Rabbit monoclonal antibody (STJ11103019) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.

Anti-CIRBP antibody (1-172) [S9MR] (STJ11103019)

SKU:
STJ11103019

£212.50 - £501.50
Shipping:
Free Shipping
Current Stock:
Host: Rabbit
Applications: WB/IHC/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit monoclonal antibody anti-CIRBP (1-172) is suitable for use in Western Blot, Immunohistochemistry and Immunofluorescence research applications.
Clonality: Monoclonal
Clone ID: S9MR
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 0.05% BSA, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:1000
IHC-P 1:50-1:200
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: CIRBP
Gene ID: 1153
Uniprot ID: CIRBP_HUMAN
Immunogen Region: 1-172
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-172 of human CIRBP (Q14011).
Immunogen Sequence: MASDEGKLFVGGLSFDTNEQ SLEQVFSKYGQISEVVVVKD RETQRSRGFGFVTFENIDDA KDAMMAMNGKSVDGRQIRVD QAGKSSDNRSRGYRGGSAGG RGFFRGGRGRGRGFSRGGGD RGYGGNRFESRSGGYGGSRD YYSSRSQSGGYSDRSSGGSY RDSYDSYATHNE
Tissue Specificity Ubiquitous.
Post Translational Modifications Methylated on arginine residues. Methylation of the RGG motifs is a prerequisite for recruitment into SGs. Phosphorylated by CK2, GSK3A and GSK3B. Phosphorylation by GSK3B increases RNA-binding activity to the TXN 3'-UTR transcript upon exposure to UV radiation.
Function Cold-inducible mRNA binding protein that plays a protective role in the genotoxic stress response by stabilizing transcripts of genes involved in cell survival. Acts as a translational activator. Seems to play an essential role in cold-induced suppression of cell proliferation. Binds specifically to the 3'-untranslated regions (3'-UTRs) of stress-responsive transcripts RPA2 and TXN. Acts as a translational repressor. Promotes assembly of stress granules (SGs), when overexpressed.
Protein Name Cold-Inducible Rna-Binding Protein
A18 Hnrnp
Glycine-Rich Rna-Binding Protein Cirp
Cellular Localisation Nucleus
Nucleoplasm
Cytoplasm
Translocates From The Nucleus To The Cytoplasm After Exposure To Uv Radiation
Translocates From The Nucleus To The Cytoplasm Into Stress Granules Upon Various Cytoplasmic Stresses
Such As Osmotic And Heat Shocks
Its Recruitment Into Stress Granules Occurs In The Absence Of Tiar Proteins
Alternative Antibody Names Anti-Cold-Inducible Rna-Binding Protein antibody
Anti-A18 Hnrnp antibody
Anti-Glycine-Rich Rna-Binding Protein Cirp antibody
Anti-CIRBP antibody
Anti-A18HNRNP antibody
Anti-CIRP antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance