Anti-Chrnb4 antibody (Cytoplasmic Loop) (STJA0003704)
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
ImmunogenFusion protein from the cytoplasmic loop of the beta 4 subunit of rat nAChR.
General Information
| Short Description | Rabbit polyclonal anti-nAChR b4 (Cytoplasmic Loop) for use in WB in Mouse and Rat samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB |
| Host | Rabbit |
| Reactivity | Mouse/Rat |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Purification | This antibody was antigen affinity purified from pooled serum. |
| Dilution Range | WB 1:1000 |
| Formulation | 100 ยตl in 10 mM HEPES (pH 7.5) , 150 mM NaCl, 100 ยตg per ml BSA and 50% Glycerol. |
| Storage Instruction | Store at-20ยฐC for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | Chrnb4 |
| Gene ID | 108015 |
| Uniprot ID | ACHB4_MOUSE |
| Immunogen | Fusion protein from the cytoplasmic loop of the beta 4 subunit of rat nAChR. |
| Immunogen Region | Cytoplasmic Loop |
Additional Info
| Tissue Specificity | Predominantly expressed by immature T-cells in the thymus. |
| Function | Component of neuronal acetylcholine receptors (nAChRs) that function as pentameric, ligand-gated cation channels with high calcium permeability among other activities. nAChRs are excitatory neurotrasnmitter receptors formed by a collection of nAChR subunits known to mediate synaptic transmission in the nervous system and the neuromuscular junction. Each nAchR subunit confers differential attributes to channel properties, including activation, deactivation and desensitization kinetics, pH sensitivity, cation permeability, and binding to allosteric modulators. CHRNB4 forms heteropentameric neuronal acetylcholine receptors with CHRNA2, CHRNA3 and CHRNA4, as well as CHRNA5 and CHRNB3 as accesory subunits. CHRNA3:CHRNB4 being predominant in neurons of the autonomic ganglia, it is known as ganglionic nicotinic receptor. CHRNA3:CHRNB4 or CHRNA3:CHRNA5:CHRNB4 play also an important role in the habenulo-interpeduncular tract, modulating the mesolimbic dopamine system and affecting reward circuits and addiction. Hypothalamic CHRNA3:CHRNB4 nAChR activation by nicotine leads to activation of POMC neurons and a decrease in food intake. |
| Protein Name | Neuronal Acetylcholine Receptor Subunit Beta-4 |
| Database Links | Reactome: R-MMU-629587Reactome: -MMU-629594Reactome: -MMU-629597Reactome: -MMU-6798695 |
| Cellular Localisation | Synaptic Cell MembraneMulti-Pass Membrane ProteinCell Membrane |
| Alternative Antibody Names | Anti-Neuronal Acetylcholine Receptor Subunit Beta-4 antibodyAnti-Chrnb4 antibodyAnti-Acrb4 antibody |
Information sourced from Uniprot.org