Anti-CHRNA4 antibody (500-600) [S5MR] (STJ11101235)
SPECIFICATIONS
ClonalityMonoclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
General Information
| Short Description | Rabbit monoclonal ACHA4 (500-600) antibody for use in WB and ELISA in human, mouse and rat samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB/ELISA |
| Host | Rabbit |
| Reactivity | Human/Mouse/Rat |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Monoclonal |
| Clone ID | S5MR |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | Lot specific |
| Purification | Affinity purification |
| Dilution Range | WB:1:1000-1:6000ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Formulation | PBS with 0.02% Sodium Azide, 0.05% BSA, 50% Glycerol, pH 7.3. |
| Storage Instruction | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | CHRNA4 |
| Gene ID | 1137 |
| Uniprot ID | ACHA4_HUMAN |
| Immunogen Region | 500-600 |
| Immunogen Sequence | PEADGQAAGALASRNTHSAE LPPPDQPSPCKCTCKKEPSS VSPSATVKTRSTKAPPPHLP LSPALTRAVEGVQYIADHLK AEDTDFSVKEDWKYVAMVID R |
| Specificity | A synthetic peptide corresponding to a sequence within amino acids 500-600 of human ACHA4 (P43681). |
Additional Info
| Function | Component of neuronal acetylcholine receptors (nAChRs) that function as pentameric, ligand-gated cation channels with high calcium permeability among other activities. nAChRs are excitatory neurotrasnmitter receptors formed by a collection of nAChR subunits known to mediate synaptic transmission in the nervous system and the neuromuscular junction. Each nAchR subunit confers differential attributes to channel properties, including activation, deactivation and desensitization kinetics, pH sensitivity, cation permeability, and binding to allosteric modulators. CHRNA4 forms heteropentameric neuronal acetylcholine receptors with CHRNB2 and CHRNB4, as well as CHRNA5 and CHRNB3 as accesory subunits. Is the most abundant nAChR subtype expressed in the central nervous system. Found in two major stoichiometric forms,(CHRNA4)3:(CHRNB2)2 and (CHRNA4)2:(CHRNB2)3, the two stoichiometric forms differ in their unitary conductance, calcium permeability, ACh sensitivity and potentiation by divalent cation. Involved in the modulation of calcium-dependent signaling pathways, influences the release of neurotransmitters, including dopamine, glutamate and GABA. |
| Protein Name | Neuronal Acetylcholine Receptor Subunit Alpha-4 |
| Database Links | Reactome: R-HSA-629587Reactome: R-HSA-629594Reactome: R-HSA-629597 |
| Cellular Localisation | Synaptic Cell MembraneMulti-Pass Membrane ProteinCell Membrane |
| Alternative Antibody Names | Anti-Neuronal Acetylcholine Receptor Subunit Alpha-4 antibodyAnti-CHRNA4 antibodyAnti-NACRA4 antibody |
Information sourced from Uniprot.org