• Anti-CHRM1 antibody (6-460) (STJ11101182)
  • Immunofluorescence analysis of NIH-3T3 cells using CHRM1 Rabbit polyclonal antibody (STJ11101182) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of C6 cells using CHRM1 Rabbit polyclonal antibody (STJ11101182) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of OVCAR-3 cells using CHRM1 Rabbit polyclonal antibody (STJ11101182) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
  • Western blot analysis of various lysates using CHRM1 Rabbit polyclonal antibody (STJ11101182) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-CHRM1 antibody (6-460) (STJ11101182)

SKU:
STJ11101182

$151.88 - $705.38
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Host: Rabbit
Applications: WB/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-CHRM1 (6-460) is suitable for use in Western Blot and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:1000-1:5000
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: CHRM1
Gene ID: 1128
Uniprot ID: ACM1_HUMAN
Immunogen Region: 6-460
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 6-460 of human CHRM1 (NP_000729.2).
Immunogen Sequence: PPAVSPNITVLAPGKGPWQ
Function The muscarinic acetylcholine receptor mediates various cellular responses, including inhibition of adenylate cyclase, breakdown of phosphoinositides and modulation of potassium channels through the action of G proteins. Primary transducing effect is Pi turnover.
Protein Name Muscarinic Acetylcholine Receptor M1
Database Links Reactome: R-HSA-390648
Reactome: R-HSA-416476
Cellular Localisation Cell Membrane
Multi-Pass Membrane Protein
Postsynaptic Cell Membrane
Alternative Antibody Names Anti-Muscarinic Acetylcholine Receptor M1 antibody
Anti-CHRM1 antibody

Information sourced from Uniprot.org

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