• Immunofluorescence analysis of THP-1 cells using CHAT antibody (STJ115210) at dilution of 1:100. Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of NIH/3T3 cells using CHAT antibody (STJ115210) at dilution of 1:100. Blue: DAPI for nuclear staining.
  • Western blot analysis of various lysates, using CHAT Rabbit polyclonal antibody (STJ115210) at 1:2500 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.

Anti-CHAT antibody (331-630) (STJ115210)

SKU:
STJ115210

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Host: Rabbit
Applications: WB/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-CHAT (331-630) is suitable for use in Western Blot and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:1000-1:2000
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: CHAT
Gene ID: 1103
Uniprot ID: CLAT_HUMAN
Immunogen Region: 331-630
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 331-630 of human CHAT (NP_065574.4).
Immunogen Sequence: TQLRKIVKMASNEDERLPPI GLLTSDGRSEWAEARTVLVK DSTNRDSLDMIERCICLVCL DAPGGVELSDTHRALQLLHG GGYSKNGANRWYDKSLQFVV GRDGTCGVVCEHSPFDGIVL VQCTEHLLKHVTQSSRKLIR ADSVSELPAPRRLRWKCSPE IQGHLASSAEKLQRIVKNLD FIVYKFDNYGKTFIKKQKCS PDAFIQVALQLAFYRLHRRL VPTYESASIRRFQEGRVDN
Function Catalyzes the reversible synthesis of acetylcholine (ACh) from acetyl CoA and choline at cholinergic synapses.
Protein Name Choline O-Acetyltransferase
Choactase
Chat
Choline Acetylase
Database Links Reactome: R-HSA-1483191
Reactome: R-HSA-264642
Alternative Antibody Names Anti-Choline O-Acetyltransferase antibody
Anti-Choactase antibody
Anti-Chat antibody
Anti-Choline Acetylase antibody
Anti-CHAT antibody

Information sourced from Uniprot.org

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