• Immunofluorescence analysis of Hela cells using CHAMP1 Rabbit polyclonal antibody (STJ113290) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
  • Western blot analysis of various lysates using CHAMP1 Rabbit polyclonal antibody (STJ113290) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.

Anti-CHAMP1 antibody (563-812) (STJ113290)

SKU:
STJ113290

£112.50 - £522.50
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Host: Rabbit
Applications: WB/IF
Reactivity: Human
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-CHAMP1 (563-812) is suitable for use in Western Blot and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IF/ICC 1:50-1:100
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: CHAMP1
Gene ID: 283489
Uniprot ID: CHAP1_HUMAN
Immunogen Region: 563-812
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 563-812 of human CHAMP1 (NP_115812.1).
Immunogen Sequence: LPKSALFSESQKAVELGDEL QIDAIDDQKCDILVQEELLA SPKKLLEDTLFPSSKKLKKD NQESSDAELSSSEYIKTDLD AMDIKGQESSSDQEQVDVES IDFSKENKMDMTSPEQSRNV LQFTEEKEAFISEEEIAKYM KRGKGKYYCKICCCRAMKKG AVLHHLVNKHNVHSPYKCTI CGKAFLLESLLKNHVAAHGQ SLLKCPRCNFESNFPRGFKK HLTHCQSRHNEEANKKLME
Post Translational Modifications Phosphorylated by CDK1. Mitotic phosphorylation is required for the attachment of spindle microtubules to the kinetochore.
Function Required for proper alignment of chromosomes at metaphase and their accurate segregation during mitosis. Involved in the maintenance of spindle microtubules attachment to the kinetochore during sister chromatid biorientation. May recruit CENPE and CENPF to the kinetochore.
Protein Name Chromosome Alignment-Maintaining Phosphoprotein 1
Zinc Finger Protein 828
Cellular Localisation Nucleus
Chromosome
Centromere
Kinetochore
Cytoplasm
Cytoskeleton
Spindle
Alternative Antibody Names Anti-Chromosome Alignment-Maintaining Phosphoprotein 1 antibody
Anti-Zinc Finger Protein 828 antibody
Anti-CHAMP1 antibody
Anti-C13orf8 antibody
Anti-CAMP antibody
Anti-CHAMP antibody
Anti-KIAA1802 antibody
Anti-ZNF828 antibody

Information sourced from Uniprot.org

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