• Immunofluorescence analysis of paraffin-embedded Human pancreas tissue using CHAC1 Rabbit polyclonal antibody (STJ118037) at a dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining. Perform high pressure antigen retrieval with 0. 01 M citrate buffer (pH 6. 0) prior to IF staining.
  • Immunohistochemistry analysis of CHAC1 in paraffin-embedded rat kidney tissue using CHAC1 Rabbit polyclonal antibody (STJ118037) at a dilution of 1:100 (40x lens). High pressure antigen retrieval was performed with 0. 01 M citrate buffer (pH 6. 0) prior to immunohistochemistry staining.
  • Immunohistochemistry analysis of CHAC1 in paraffin-embedded mouse testis tissue using CHAC1 Rabbit polyclonal antibody (STJ118037) at a dilution of 1:100 (40x lens). High pressure antigen retrieval was performed with 0. 01 M citrate buffer (pH 6. 0) prior to immunohistochemistry staining.
  • Immunohistochemistry analysis of CHAC1 in paraffin-embedded human small intestine tissue using CHAC1 Rabbit polyclonal antibody (STJ118037) at a dilution of 1:100 (40x lens). High pressure antigen retrieval was performed with 0. 01 M citrate buffer (pH 6. 0) prior to immunohistochemistry staining.
  • Western blot analysis of lysates from Mouse stomach, using CHAC1 Rabbit polyclonal antibody (STJ118037) at 1:900 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.

Anti-CHAC1 antibody (1-222) (STJ118037)

SKU:
STJ118037

£112.50 - £524.50
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Host: Rabbit
Applications: WB/IHC-P/IF/ICC/ELISA
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.05% Proclin300, 50% Glycerol, pH 7.3.
Purification: Affinity purification
Concentration: Lot specific
Dilution Range: WB:1:500-1:1000
IHC-P:1:50-1:200
IF/ICC:1:50-1:200
ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements.
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: CHAC1
Gene ID: 79094
Uniprot ID: CHAC1_HUMAN
Immunogen Region: 1-222
Specificity: Recombinant fusion protein containing a sequence corresponding to amino acids 1-222 of human CHAC1 (NP_077016.3).
Immunogen Sequence: MKQESAAPNTPPTSQSPTPS AQFPRNDGDPQALWIFGYGS LVWRPDFAYSDSRVGFVRGY SRRFWQGDTFHRGSDKMPGR VVTLLEDHEGCTWGVAYQVQ GEQVSKALKYLNVREAVLGG YDTKEVTFYPQDAPDQPLKA LAYVATPQNPGYLGPAPEEA IATQILACRGFSGHNLEYLL RLADFMQLCGPQAQDEHLAA IVDAVGTMLPCFCPTEQALA LV
Function Catalyzes the cleavage of glutathione into 5-oxo-L-proline and a Cys-Gly dipeptide. Acts specifically on glutathione, but not on other gamma-glutamyl peptides. Glutathione depletion is an important factor for apoptosis initiation and execution. Acts as a pro-apoptotic component of the unfolded protein response pathway by mediating the pro-apoptotic effects of the ATF4-ATF3-DDIT3/CHOP cascade. Negative regulator of Notch signaling pathway involved in embryonic neurogenesis: acts by inhibiting Notch cleavage by furin, maintaining Notch in an immature inactive form, thereby promoting neurogenesis in embryos.
Protein Name Glutathione-Specific Gamma-Glutamylcyclotransferase 1
Gamma-Gcg 1
Blocks Notch Protein
Botch
Cation Transport Regulator-Like Protein 1
Database Links Reactome: R-HSA-174403
Reactome: R-HSA-9648895
Cellular Localisation Cytoplasm
Cytosol
Golgi Apparatus
Trans-Golgi Network
Alternative Antibody Names Anti-Glutathione-Specific Gamma-Glutamylcyclotransferase 1 antibody
Anti-Gamma-Gcg 1 antibody
Anti-Blocks Notch Protein antibody
Anti-Botch antibody
Anti-Cation Transport Regulator-Like Protein 1 antibody
Anti-CHAC1 antibody
Anti-BOTCH antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance